ArticleNature ecology & evolution2024
Single-cell analysis of the amphioxus hepatic caecum and vertebrate liver reveals genetic mechanisms of vertebrate liver evolution.
Article in Nature ecology & evolution, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- Systematic benchmarking and optimal strategy selection of cross-species integration methods.Briefings in bioinformatics · 2026Article
- JMJD3-mediated HADH demethylation orchestrates hepatocyte maturation through fatty acid β-oxidation-dependent histone acetylation.Experimental & molecular medicine · 2026Article
- Apolipoprotein D neofunctionalization couples lipid allocation to wing evolution.The EMBO journal · 2026Article
- The molecular evolution of vertebrate organs.Nature ecology & evolution · 2026Review
- Spontaneous whole genome duplication renders mouse embryonic fibroblasts resistant to reprogramming.Cell & bioscience · 2026Article
- Chromosome-level genome assembly of masu salmon (Oncorhynchus masou masou).Scientific data · 2026Article
- scGeneBank: cross-species screening of functional gene sets at single-cell resolution.Nucleic acids research · 2026Article
- Single cell and single nucleus RNA sequencing in liver tissues: applications and prospects in model and non-model organisms.Frontiers in genetics · 2026Review
- Comparative transcriptomics reveal the common anteroposterior molecular blueprint of adult bilaterian guts.PLoS biology · 2026Article
- Near complete T2T genome assembly of the banded goonch (Bagarius rutilus).Scientific data · 2025Article
- Single nucleus RNA sequencing profile analysis to reveal cell type specific common molecular drivers of Parkinson's disease and therapeutic agents.Scientific reports · 2025Article
- Targeted screening and single-cell analysis of genetic variants in melanoma using Mendelian randomization.Discover oncology · 2025Article
- Haplotype resolved chromosome-level genome assembly of the gold barb (Barbodes semifasciolatus).Scientific data · 2025Article
- Origin and stepwise evolution of vertebrate lungs.Nature ecology & evolution · 2025Article
- The genome sequence of the amphioxus,Wellcome open research · 2025Article
- Looking back on 2024.Nature ecology & evolution · 2025Article
- Metabolic complementation between cells drives the evolution of tissues and organs.Biology letters · 2024Article
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20 authors.
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Abstract
The evolution of the vertebrate liver is a prime example of the evolution of complex organs, yet the driving genetic factors behind it remain unknown. Here we study the evolutionary genetics of liver by comparing the amphioxus hepatic caecum and the vertebrate liver, as well as examining the functional transition within vertebrates. Using in vivo and in vitro experiments, single-cell/nucleus RNA-seq data and gene knockout experiments, we confirm that the amphioxus hepatic caecum and vertebrate liver are homologous organs and show that the emergence of ohnologues from two rounds of whole-genome duplications greatly contributed to the functional complexity of the vertebrate liver. Two ohnologues, kdr and flt4, play an important role in the development of liver sinusoidal endothelial cells. In addition, we found that liver-related functions such as coagulation and bile production evolved in a step-by-step manner, with gene duplicates playing a crucial role. We reconstructed the genetic footprint of the transfer of haem detoxification from the liver to the spleen during vertebrate evolution. Together, these findings challenge the previous hypothesis that organ evolution is primarily driven by regulatory elements, underscoring the importance of gene duplicates in the emergence and diversification of a complex organ.
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