ArticleJournal of industrial microbiology & biotechnology2024
Optimizing dsRNA engineering strategies and production in E. coli HT115 (DE3).
Article in Journal of industrial microbiology & biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The trial behind it
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Who cites it
9 citing papers in PubMed.
- Efficient control of Tribulium castaneum using zeolitic imidazolate framework-8 nanocarrier-mediated delivery of dsRNA targeting Paramyosin.Pest management science · 2026Article
- Genetic Biosensor for Optimizing Double-Stranded RNA Production by Bacteria.ACS synthetic biology · 2026Article
- RNA interference in crop protection: opportunities and challenges during the transition to commercialization.Pest management science · 2026Review
- Comparison of T7 In Vitro Transcription andInsects · 2026Article
- Programmed mitophagy at the oocyte-to-zygote transition promotes lineage endurance.Nature cell biology · 2026Article
- Control Strategies of Plant Viruses Using Spray-Induced Gene Silencing.The plant pathology journal · 2026Review
- Harnessing double-stranded RNA (dsRNA): a sustainable approach to pest management.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- RNAi spray-induced gene silencing ofFrontiers in plant science · 2025Article
- Spray-induced gene silencing for crop protection: recent advances and emerging trends.Frontiers in plant science · 2025Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Producing double-stranded RNA (dsRNA) represents a bottleneck for the adoption of RNA interference technology in agriculture, and the main hurdles are related to increases in dsRNA yield, production efficiency, and purity. Therefore, this study aimed to optimize dsRNA production in E. coli HT115 (DE3) using an in vivo system. To this end, we designed a new vector, pCloneVR_2, which resulted in the efficient production of dsRNA in E. coli HT115 (DE3). We performed optimizations in the culture medium and expression inducer in the fermentation of E. coli HT115 (DE3) for the production of dsRNA. Notably, the variable that had the greatest effect on dsRNA yield was cultivation in TB medium, which resulted in a 118% increase in yield. Furthermore, lactose induction (6 g/L) yielded 10 times more than IPTG. Additionally, our optimized up-scaled protocol of the TRIzol™ extraction method was efficient for obtaining high-quality and pure dsRNA. Finally, our optimized protocol achieved an average yield of 53.3 µg/mL after the production and purification of different dsRNAs, reducing production costs by 72%.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.