Evidence map›Paper›PMID 39151705›Full record

ArticleVirologica Sinica2024

Identification of mutations in viral proteins involved in cell adaptation using a reverse genetic system of the live attenuated hepatitis A virus vaccine H2 strain.

Xiu-Li Yan, Jian Li, Qing-Qing Ma, Hong-Jiang Wang, Lin Li, Hui Zhao, Cheng-Feng Qin, Xiao-Feng Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virologica Sinica, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xiu-Li YanSchool of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui, 230032, China; Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China.
Jian LiDepartment of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China; School of Medicine, Tsinghua University, Beijing, 100084, China.
Qing-Qing MaDepartment of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China.
Hong-Jiang WangDepartment of Research, The Chinese People's Liberation Army Strategic Support Force Medical Center, Beijing, 100101, China.
Lin LiDepartment of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China; School of Medicine, Tsinghua University, Beijing, 100084, China.
Hui ZhaoDepartment of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China.
Cheng-Feng QinDepartment of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China. Electronic address: qincf@bmi.ac.cn.
Xiao-Feng LiSchool of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui, 230032, China; Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China. Electronic address: xiaofeng_li_bj@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The live attenuated hepatitis A virus vaccine H2 strain was developed by passaging a wild-type H2w isolate in cell cultures. Currently, the mechanism underlying its attenuation phenotype remain largely unknown. In this study, we generated a full-length infectious cDNA clone of the H2 strain using in-fusion techniques. The recovered H2 strain (H2ic) from the cDNA clone exhibited an efficient replication in both the hepatoma cell line Huh7.5.1 and the 2BS cell line used for vaccine production, similar to the parental H2 strain. Additionally, H2ic did not cause disease in Ifnar1

Indexed as

Hepatitis A virusMice, Inbred C57BLMutationVaccines, AttenuatedVirus ReplicationAdaptation, BiologicalAnimalsCell LineHepatitis AHumansMiceMice, KnockoutReverse GeneticsViral Hepatitis VaccinesViral ProteinsVaccines, AttenuatedViral Hepatitis VaccinesViral ProteinsCell adaptive mutationsHepatitis A virus (HAV)Live attenuated hepatitis A virus vaccine H2 strainReverse genetics

Identifiers

PMID39151705
PMCPMC11738778

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.