Evidence map›Paper›PMID 39150484›Full record

ArticleAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry2024

Enzyme-aided amplification strategy for sensitive detection of methamphetamine based on fluorescence aptamer sensor.

Zheyu Wang, Yandan Wang, Yishuo Tong, Weifen Niu

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Article in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Zheyu Wang *School of Forensic Medicine, Key Laboratory of Forensic Toxicology of Ministry of Public Security, Shanxi Medical University, Jinzhong, 030600, Shanxi, People's Republic of China.
Yandan Wang *School of Forensic Medicine, Key Laboratory of Forensic Toxicology of Ministry of Public Security, Shanxi Medical University, Jinzhong, 030600, Shanxi, People's Republic of China.
Yishuo TongSchool of Forensic Medicine, Key Laboratory of Forensic Toxicology of Ministry of Public Security, Shanxi Medical University, Jinzhong, 030600, Shanxi, People's Republic of China.
Weifen NiuSchool of Forensic Medicine, Key Laboratory of Forensic Toxicology of Ministry of Public Security, Shanxi Medical University, Jinzhong, 030600, Shanxi, People's Republic of China. niuweifen_2000@163.com.ORCID http://orcid.org/0000-0002-7526-2393

Funding

Natural Science Foundation of Shanxi Province, China 20210302123303
6 · The paper itself

Abstract

Methamphetamine (METH) abuse poses a serious risk to human health and social stability. It is critical to develop sensitive and selective methods for detecting METH. Here, we develop a fluorescence aptamer sensor to detect METH based on DNA exonuclease III (Exo III), graphene oxide (GO), and FAM-labeled aptamer. First, the sensor used GO's strong binding capacity to adsorb and quench the fluorescence of the aptamer attached to GO surface. When METH was added to the system, the formation of stable complex for aptamer and METH dissociated from the surface of GO, leading to a fluorescence restoration. Then, the fluorescence signal was further amplified by using Exo III to liberate target METH for cyclic hybridization. And the gel electrophoresis experiment further verified the reliability of this strategy. This aptamer sensor exhibited a low detection limit (0.52 nM) and excellent selectivity under optimal conditions. Notably, this sensor has been successfully validated in the detection of METH in urine and saliva samples, exhibited commendable recovery (94.00-104.65%). Its benefits include facile, sensitive, and rapid. Expected to be used in practical METH detection.

Indexed as

Aptamers, NucleotideExodeoxyribonucleasesGraphiteMethamphetamineBiosensing TechniquesFluorescenceHumansLimit of DetectionSpectrometry, FluorescenceAptamers, Nucleotideexodeoxyribonuclease IIIExodeoxyribonucleasesgraphene oxideGraphiteMethamphetamineAptamerExonuclease III (Exo III)Fluorescence sensorGraphene oxide (GO)Methamphetamine (METH)

Identifiers

PMID39150484

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.