Evidence map›Paper›PMID 39150461›Full record

ArticleCurrent genetics2024

Deletions of ttrA and pduA genes in Salmonella enterica affect survival within chicken-derived HD-11 macrophages.

Mauro M S Saraiva, Valdinete P Benevides, Priscila R Guerra, Isabella C Campos, Lucas B Rodrigues Alves, Jacqueline B Paiva, Lauanda M Muniz, Adriana M Almeida, Oliveiro C Freitas Neto, John E Olsen and 1 more

Abstract read
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In one paragraph

Article in Current genetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. A double ttrA and pduA knock-out mutant of Salmonella Typhimurium is not attenuated for mice (Mus musculus).Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Mauro M S Saraiva *School of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, 14884-900, Brazil. saraiva_ufba@hotmail.com.ORCID http://orcid.org/0000-0003-1875-4495
Valdinete P Benevides *School of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, 14884-900, Brazil.ORCID http://orcid.org/0000-0002-7552-1607
Priscila R GuerraDepartment of Veterinary and Animal Sciences, University of Copenhagen (UK), Frederiksberg C., 1870, Denmark.ORCID http://orcid.org/0000-0002-1082-6004
Isabella C CamposSchool of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, 14884-900, Brazil.ORCID http://orcid.org/0000-0002-9384-6085
Lucas B Rodrigues AlvesSchool of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, 14884-900, Brazil.ORCID http://orcid.org/0000-0001-5788-5514
Jacqueline B PaivaR&D Department, BioCamp Laboratories Ltda, Campinas, SP, 13082-020, Brazil.ORCID http://orcid.org/0000-0002-4844-0895
Lauanda M MunizR&D Department, BioCamp Laboratories Ltda, Campinas, SP, 13082-020, Brazil.ORCID http://orcid.org/0009-0003-5136-5210
Adriana M AlmeidaSchool of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, 14884-900, Brazil.ORCID http://orcid.org/0000-0002-7264-1734
Oliveiro C Freitas NetoDepartment of Preventive Veterinary Medicine, Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, 31279-901, Brazil.ORCID http://orcid.org/0000-0002-1437-069X
John E OlsenDepartment of Veterinary and Animal Sciences, University of Copenhagen (UK), Frederiksberg C., 1870, Denmark.ORCID http://orcid.org/0000-0001-6225-6587
Angelo Berchieri JuniorSchool of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, 14884-900, Brazil. angelo.berchieri@unesp.br.ORCID http://orcid.org/0000-0003-2522-6500

Funding

Fundação de Amparo à Pesquisa do Estado de São Paulo 2018/03189-0Fundação de Amparo à Pesquisa do Estado de São Paulo 2018/21301-2
6 · The paper itself

Abstract

In mammals, enteric salmonellas can use tetrathionate (ttr), formed as a by-product from the inflammatory process in the intestine, as electron acceptor in anaerobic respiration, and it can fuel its energy metabolism by degrading the microbial fermentation product 1,2-propanediol. However, recent studies have shown that this mechanism is not important for Salmonella infection in the intestine of poultry, while it prolongs the persistence of Salmonella at systemic sites in this species. In the current study, we show that ΔttrApduA strains of Salmonella enterica have lower net survival within chicken-derived HD-11 macrophages, as CFU was only 2.3% (S. Enteritidis ΔttrApduA), 2.3% (S. Heidelberg ΔttrApduA), and 3.0% (S. Typhimurium ΔttrApduA) compared to wild-type strains after 24 h inside HD-11 macrophage cells. The difference was not related to increased lysis of macrophages, and deletion of ttrA and pduA did not impair the ability of the strains to grow anaerobically. Further studies are indicated to determine the reason why Salmonella ΔttrApduA strains survive less well inside macrophage cell lines.

Indexed as

ChickensMacrophagesSalmonella entericaAnimalsBacterial ProteinsCell LineGene DeletionMicrobial ViabilitySalmonella Infections, AnimalBacterial Proteins1,2-propanediolMacrophageSalmonella EnteritidisSalmonella HeidelbergSalmonella TyphimuriumTetrathionate

Identifiers

PMID39150461

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.