Evidence map›Paper›PMID 39149770›Full record

ArticleAdvanced materials (Deerfield Beach, Fla.)2024

Polymeric Nanocarriers Autonomously Cross the Plant Cell Wall and Enable Protein Delivery for Stress Sensing.

Yilin Zhang, Yunteng Cao, Wenzhi Jiang, Qingquan Ma, Jinwoo Shin, Hui Sun, Jianqiao Cui, Yongsheng Chen, Juan Pablo Giraldo, Michael S Strano and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced materials (Deerfield Beach, Fla.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Molecular aspects of heat stress sensing in land plants.The Plant journal : for cell and molecular biology · 2025
    Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yilin ZhangDepartment of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.ORCID 0000-0003-4246-3620
Yunteng CaoDepartment of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.ORCID 0000-0002-6425-7628
Wenzhi JiangDepartment of Molecular Biology and Centre for Computational and Integrative Biology, Department of Genetics, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
Qingquan MaSchool of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA, 30332, USA.ORCID 0000-0001-6879-6410
Jinwoo ShinDepartment of Molecular Biology and Centre for Computational and Integrative Biology, Department of Genetics, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
Hui SunDepartment of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.ORCID 0000-0003-1831-2750
Jianqiao CuiDepartment of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
Yongsheng ChenSchool of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA, 30332, USA.ORCID 0000-0002-9519-2302
Juan Pablo GiraldoDepartment of Botany and Plant Sciences, University of California, Riverside, CA, 92521, USA.ORCID 0000-0002-8400-8944
Michael S StranoDepartment of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.ORCID 0000-0003-2944-808X
Gregory V LowryDepartment of Civil and Environmental Engineering, Carnegie Mellon University, Pittsburgh, PA, 15213, USA.ORCID 0000-0001-8599-008X
Jen SheenDepartment of Molecular Biology and Centre for Computational and Integrative Biology, Department of Genetics, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
Benedetto MarelliDepartment of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.ORCID 0000-0001-5311-6961

Funding

Plant Nutrient-Growth Signaling NetworkR01GM129093 · NIGMS · MASSACHUSETTS GENERAL HOSPITAL · PI SHEEN, JEN · 2018 to 2025
$2.8M
Campus for Research Excellence and Technological EnterpriseCREATEDisruptive & Sustainable Technology for Agricultural PrecisionDiSTAPFMSG 2134535NIGMS NIH HHS R01 GM129093NIH HHS GM129093NSF FMSG:BIO:2134535NSF CMMI 1752172Office of Naval Research DURIP N000142012203ONR DURIP N000142012203Singapore-MIT Alliance for Research and Technology Centre
6 · The paper itself

Abstract

Delivery of proteins in plant cells can facilitate the design of desired functions by modulation of biological processes and plant traits but is currently limited by narrow host range, tissue damage, and poor scalability. Physical barriers in plants, including cell walls and membranes, limit protein delivery to desired plant tissues. Herein, a cationic high aspect ratio polymeric nanocarriers (PNCs) platform is developed to enable efficient protein delivery to plants. The cationic nature of PNCs binds proteins through electrostatic. The ability to precisely design PNCs' size and aspect ratio allowed us to find a cutoff of ≈14 nm in the cell wall, below which cationic PNCs can autonomously overcome the barrier and carry their cargo into plant cells. To exploit these findings, a reduction-oxidation sensitive green fluorescent protein (roGFP) is deployed as a stress sensor protein cargo in a model plant Nicotiana benthamiana and common crop plants, including tomato and maize. In vivo imaging of PNC-roGFP enabled optical monitoring of plant response to wounding, biotic, and heat stressors. These results show that PNCs can be precisely designed below the size exclusion limit of cell walls to overcome current limitations in protein delivery to plants and facilitate species-independent plant engineering.

Indexed as

Cell WallGreen Fluorescent ProteinsPolymersDrug CarriersNanoparticlesNicotianaStress, PhysiologicalZea maysDrug CarriersGreen Fluorescent ProteinsPolymersnanocarrierplant engineeringpolymersprotein deliverystress sensing

Identifiers

PMID39149770
PMCPMC11466712

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.