Evidence map›Paper›PMID 39149402›Full record

ArticlebioRxiv : the preprint server for biology2024

A synthetic cell-free pathway for biocatalytic upgrading of one-carbon substrates.

Grant M Landwehr, Bastian Vogeli, Cong Tian, Bharti Singal, Anika Gupta, Rebeca Lion, Edward H Sargent, Ashty S Karim, Michael C Jewett

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Grant M LandwehrDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, IL, 60208, USA.
Bastian VogeliDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, IL, 60208, USA.
Cong TianDepartment of Chemistry, Northwestern University, Evanston, IL, 60208, USA.
Bharti SingalStanford SLAC CryoEM Initiative, Stanford University; Stanford, CA 94305, USA.
Anika GuptaDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, IL, 60208, USA.
Rebeca LionDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, IL, 60208, USA.
Edward H SargentDepartment of Chemistry, Northwestern University, Evanston, IL, 60208, USA.
Ashty S KarimDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, IL, 60208, USA.
Michael C JewettDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, IL, 60208, USA.

Funding

ChimeraX -- Next Generation Visualization and Analysis Software for Multiscale ModelingR01GM129325 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIN, THOMAS E · 2018 to 2025
$5.2M
NIGMS NIH HHS R01 GM129325
6 · The paper itself

Abstract

Biotechnological processes hold tremendous potential for the efficient and sustainable conversion of one-carbon (C1) substrates into complex multi-carbon products. However, the development of robust and versatile biocatalytic systems for this purpose remains a significant challenge. In this study, we report a hybrid electrochemical-biochemical cell-free system for the conversion of C1 substrates into the universal biological building block acetyl-CoA. The synthetic reductive formate pathway (ReForm) consists of five core enzymes catalyzing non-natural reactions that were established through a cell-free enzyme engineering platform. We demonstrate that ReForm works in a plug-and-play manner to accept diverse C1 substrates including CO

Identifiers

PMID39149402
PMCPMC11326285

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.