Evidence map›Paper›PMID 39149298›Full record

ArticlebioRxiv : the preprint server for biology2024

Human Vascularized Macrophage-Islet Organoids to Model Immune-Mediated Pancreatic β cell Pyroptosis upon Viral Infection.

Liuliu Yang, Yuling Han, Tuo Zhang, Xue Dong, Jian Ge, Aadita Roy, Jiajun Zhu, Tiankun Lu, J Jeya Vandana, Neranjan de Silva and 12 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

22 authors.

Liuliu YangDepartment of Surgery, Weill Cornell Medicine, 1300 York Ave, New York, NY, 10065, USA.
Yuling HanDepartment of Surgery, Weill Cornell Medicine, 1300 York Ave, New York, NY, 10065, USA.
Tuo ZhangGenomic Resource Core Facility, Weill Cornell Medicine, New York, NY 10065, USA.
Xue DongDepartment of Surgery, Weill Cornell Medicine, 1300 York Ave, New York, NY, 10065, USA.
Jian GeColumbia Center for Human Development, Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA.
Aadita RoyDepartment of Surgery, Weill Cornell Medicine, 1300 York Ave, New York, NY, 10065, USA.
Jiajun ZhuDepartment of Surgery, Weill Cornell Medicine, 1300 York Ave, New York, NY, 10065, USA.
Tiankun LuDepartment of Surgery, Weill Cornell Medicine, 1300 York Ave, New York, NY, 10065, USA.
J Jeya VandanaDepartment of Surgery, Weill Cornell Medicine, 1300 York Ave, New York, NY, 10065, USA.ORCID 0000-0002-1026-569X
Neranjan de SilvaDepartment of Surgery, Weill Cornell Medicine, 1300 York Ave, New York, NY, 10065, USA.
Catherine C RobertsonDepartment of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USA.
Jenny Z XiangGenomic Resource Core Facility, Weill Cornell Medicine, New York, NY 10065, USA.
Chendong PanGenomic Resource Core Facility, Weill Cornell Medicine, New York, NY 10065, USA.
Yanjie SunGenomic Resource Core Facility, Weill Cornell Medicine, New York, NY 10065, USA.
Jianwen QueColumbia Center for Human Development, Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA.
Todd EvansDepartment of Surgery, Weill Cornell Medicine, 1300 York Ave, New York, NY, 10065, USA.
Chengyang LiuDepartment of Surgery, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.
Wei WangDepartment of Surgery, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.
Ali NajiDepartment of Surgery, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.
Stephen C J ParkerDepartment of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USA.
Robert E SchwartzDivision of Gastroenterology and Hepatology, Department of Medicine, Weill Cornell Medicine, 1300 York Ave, New York, NY, 10065, USA.
Shuibing ChenDepartment of Surgery, Weill Cornell Medicine, 1300 York Ave, New York, NY, 10065, USA.

Funding

The Human Islet Distribution Coordinating Center (UC4)UC4DK098085 · NIDDK · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · PI EVANS-MOLINA, CARMELLA, NILAND, JOYCE CAROL · 2012 to 2017
$25.6M
Penn integrated Human Pancreas procurement and Analysis ProgramUC4DK112217 · NIDDK · UNIVERSITY OF PENNSYLVANIA · PI BETTS, MICHAEL R, FELDMAN, MICHAEL D · 2016 to 2020
$17.8M
Abnormal Extracellular Vesicles and Particles from Human Islets Impact T1D progressionR01DK137517 · NIDDK · WEILL MEDICAL COLL OF CORNELL UNIV · PI Shuibing Chen, DAVID CHARLES LYDEN · 2023 to 2026
$2.8M
Optimization of the engineered 3D hepatic microenvironment enhances pluripotent stem cell derived hepatocyteR01DK121072 · NIDDK · WEILL MEDICAL COLL OF CORNELL UNIV · PI SCHWARTZ, ROBERT E · 2020 to 2024
$2.6M
A High Content Chemical Screen to Identify the Drug Candidates Promoting Human Beta Cell ProliferationR01DK124463 · NIDDK · WEILL MEDICAL COLL OF CORNELL UNIV · PI CHEN, SHUIBING · 2020 to 2023
$1.7M
Decode the Impact of SARS-CoV-2 on Human PancreasR01DK130454 · NIDDK · WEILL MEDICAL COLL OF CORNELL UNIV · PI CHEN, SHUIBING, LIM, JEAN KYOU · 2021 to 2023
$1.3M
A JEM 1400 Electron Microscope for a Core FacilityS10RR027699 · NCRR · WEILL MEDICAL COLL OF CORNELL UNIV · PI MAXFIELD, FREDERICK R. · 2010 to 2010
$378k
NCRR NIH HHS S10 RR027699NIDDK NIH HHS R01 DK121072NIDDK NIH HHS R01 DK124463NIDDK NIH HHS R01 DK130454NIDDK NIH HHS R01 DK137517NIDDK NIH HHS UC4 DK098085NIDDK NIH HHS UC4 DK112217
6 · The paper itself

Abstract

There is a paucity of human models to study immune-mediated host damage. Here, we utilized the GeoMx spatial multi-omics platform to analyze immune cell changes in COVID-19 pancreatic autopsy samples, revealing an accumulation of proinflammatory macrophages. Single cell RNA-seq analysis of human islets exposed to SARS-CoV-2 or Coxsackievirus B4 (CVB4) viruses identified activation of proinflammatory macrophages and β cell pyroptosis. To distinguish viral versus proinflammatory macrophage-mediated β cell pyroptosis, we developed human pluripotent stem cell (hPSC)-derived vascularized macrophage-islet (VMI) organoids. VMI organoids exhibited enhanced marker expression and function in both β cells and endothelial cells compared to separately cultured cells. Notably, proinflammatory macrophages within VMI organoids induced β cell pyroptosis. Mechanistic investigations highlighted TNFSF12-TNFRSF12A involvement in proinflammatory macrophage-mediated β cell pyroptosis. This study established hPSC-derived VMI organoids as a valuable tool for studying immune cell-mediated host damage and uncovered mechanism of β cell damage during viral exposure.

Identifiers

PMID39149298
PMCPMC11326194

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.