Evidence map›Paper›PMID 39149227›Full record

ArticlebioRxiv : the preprint server for biology2024

Integrated Single-Cell Multiomic Profiling of Caudate Nucleus Suggests Key Mechanisms in Alcohol Use Disorder.

Nick Green, Hongyu Gao, Xiaona Chu, Qiuyue Yuan, Patrick McGuire, Dongbing Lai, Guanglong Jiang, Xiaoling Xuei, Jill L Reiter, Julia Stevens and 11 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

21 authors.

Nick GreenIndiana University School of Medicine, Department of Medical and Molecular Genetics, Indianapolis, Indiana 46202, United States.
Hongyu GaoIndiana University School of Medicine, Department of Medical and Molecular Genetics, Indianapolis, Indiana 46202, United States.
Xiaona ChuIndiana University School of Medicine, Department of Medical and Molecular Genetics, Indianapolis, Indiana 46202, United States.
Qiuyue YuanClemson University, Department of Genetics and Biochemistry, Greenwood, SC 29646, United States.
Patrick McGuireIndiana University School of Medicine, Department of Medical and Molecular Genetics, Indianapolis, Indiana 46202, United States.
Dongbing LaiIndiana University School of Medicine, Department of Medical and Molecular Genetics, Indianapolis, Indiana 46202, United States.
Guanglong JiangIndiana University School of Medicine, Department of Medical and Molecular Genetics, Indianapolis, Indiana 46202, United States.
Xiaoling XueiIndiana University School of Medicine, Department of Medical and Molecular Genetics, Indianapolis, Indiana 46202, United States.
Jill L ReiterIndiana University School of Medicine, Department of Medical and Molecular Genetics, Indianapolis, Indiana 46202, United States.
Julia StevensNew South Wales Brain Tissue Research Centre, Charles Perkins Centre and School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia.
Greg T SutherlandNew South Wales Brain Tissue Research Centre, Charles Perkins Centre and School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia.
Alison M GoateNash Family Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, New York 10029, United States.ORCID 0000-0002-0576-2472
Zhiping P PangHuman Genetics Institute, Rutgers University, Piscataway, New Jersey 08854, United States.ORCID 0000-0002-6183-1233
Paul A SlesingerNash Family Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, New York 10029, United States.
Ronald P HartHuman Genetics Institute, Rutgers University, Piscataway, New Jersey 08854, United States.ORCID 0000-0003-4836-8712
Jay A TischfieldDepartment of Genetics, Rutgers University, Piscataway, New Jersey 08854, United States.
Arpana AgrawalDepartment of Psychiatry, Washington University School of Medicine in St. Louis, St. Louis, Missouri 64110, United States.
Yue WangIndiana University School of Medicine, Department of Medical and Molecular Genetics, Indianapolis, Indiana 46202, United States.
Zhana DurenIndiana University School of Medicine, Department of Medical and Molecular Genetics, Indianapolis, Indiana 46202, United States.
Howard J EdenbergIndiana University School of Medicine, Department of Medical and Molecular Genetics, Indianapolis, Indiana 46202, United States.
Yunlong LiuIndiana University School of Medicine, Department of Medical and Molecular Genetics, Indianapolis, Indiana 46202, United States.ORCID 0000-0002-2699-626X

Funding

Subject CollectionU10AA008401 · NIAAA · SUNY DOWNSTATE MEDICAL CENTER · PI JAY Arnold TISCHFIELD · 1989 to 2026
$162.7M
Research Education ComponentP30AG066514 · NIA · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI Margaret Sewell · 2020 to 2026
$31.0M
Brain Tissue Resource Centre for Alcohol ResearchR28AA012725 · NIAAA · UNIVERSITY OF SYDNEY · PI Greg Trevor Sutherland · 2012 to 2026
$7.0M
NIAAA NIH HHS R28 AA012725NIAAA NIH HHS U10 AA008401NIA NIH HHS P30 AG066514
6 · The paper itself

Abstract

Alcohol use disorder (AUD) induces complex transcriptional and regulatory changes across multiple brain regions including the caudate nucleus, which remains understudied. Using paired single-nucleus RNA-seq and ATAC-seq on caudate samples from 143 human postmortem brains, including 74 with AUD, we identified 17 distinct cell types. We found that a significant portion of the alcohol-induced changes in gene expression occurred through altered chromatin accessibility. Notably, we identified novel transcriptional and chromatin accessibility differences in medium spiny neurons, impacting pathways such as RNA metabolism and immune response. A small cluster of D1/D2 hybrid neurons showed distinct differences, suggesting a unique role in AUD. Microglia exhibited distinct activation states deviating from classical M1/M2 designations, and astrocytes entered a reactive state partially regulated by

Identifiers

PMID39149227
PMCPMC11326171

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.