Evidence map›Paper›PMID 39148630›Full record

ArticleFrontiers in molecular biosciences2024

Application of bio-layer interferometry for the analysis of ribosome-protein interactions.

Ilamathy Pandiarajan, Sujata B Walunj, Nirjhar Banerjee, Janmejaya Rout, Sanjeeva Srivastava, Swati Patankar, Sandip Kaledhonkar

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in molecular biosciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ilamathy PandiarajanDepartment of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India.
Sujata B WalunjDepartment of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India.
Nirjhar BanerjeeDepartment of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India.
Janmejaya RoutDepartment of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India.
Sanjeeva SrivastavaDepartment of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India.
Swati PatankarDepartment of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India.
Sandip KaledhonkarDepartment of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ribosome, a ribonucleoprotein complex, performs the function of protein translation. While ribosomal RNA catalyzes polypeptide formation, several proteins assist the ribosome throughout the translation process. Studying the biochemical and kinetic properties of these proteins interacting with the ribosome is vital for elucidating their roles. Various techniques, such as zonal centrifugation, pull-down assays, dynamic light scattering (DLS), fluorescence polarization, and surface plasmon resonance (SPR) are employed for this purpose, each presenting unique advantages and limitations. We add to the repertoire of techniques by using Bio-Layer Interferometry (BLI) to examine interactions between the ribosome and translation factors. Our findings demonstrate that BLI can detect interactions of

Indexed as

binding affinitybio-layer interferometryribosomeribosome-protein interactiontranslation factors

Identifiers

PMID39148630
PMCPMC11325027

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.