ArticleNature protocols2024
Optimizing the standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes.
Article in Nature protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
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- Resolving the issue of non-specific substrate oxidation in nanozyme-assisted colorimetric assays.Analytical and bioanalytical chemistry · 2026Article
- Physical Processing Controls the Structure, Mesoporosity, and Suspension-Phase Functionality of Nanostructured MnONanomaterials (Basel, Switzerland) · 2026Article
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- Colorimetric and photothermal dual-mode sensing platform for catecholamines detection based on an irregular octahedral CoFe bimetallic organic framework nanozyme.Mikrochimica acta · 2026Article
- Advances in ultrasound-mediated nanozyme systems in therapeutic applications.Ultrasonics sonochemistry · 2026Review
- Controlling pH-Dependent Nanozyme Activity by Metal Identity in Histidine-Based Nanoarchitectures.Nanomaterials (Basel, Switzerland) · 2026Article
- Standardized assays for evaluating superoxide dismutase-like and catalase-like activities of nanozymes.Nature protocols · 2026Review
- Recent advances in engineered nanozymes for precision lung cancer therapy.Materials today. Bio · 2026Review
- Deciphering the Catalytic Mechanism of Oxidoreductase-Like Nanozymes Along the Reaction Pathway: Activity, Specificity and Sustainability.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- Portable kits based on L-arginine modified Cu-CuFeMikrochimica acta · 2026Article
- Eco-Nanozymology: A Catalytic Paradigm Integrating Energy, Environment, and Ecology.Nano-micro letters · 2026Review
- Moving Beyond Fenton-Based Peroxidase Nanozymes for Analytical Sensing: Perspectives on Nonradical Alternatives from Heterogeneous Catalysis.Analytical chemistry · 2026Review
- Glucose coated FeO@FeScientific reports · 2026Article
- Can nanozymes achieve more than enzymes?Nature reviews. Materials · 2026Article
- Phase-engineered MoSJournal of nanobiotechnology · 2026Article
- Single-atom Co-N-C nanozyme with enhanced oxidase-like activity for smartphone-assisted colorimetric detection of sulfite.Mikrochimica acta · 2026Article
- Peroxidase-Mimicking Nanozymes for Rapid Detection of Infectious Diseases.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
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Authors and funding
9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Nanozymes are nanomaterials with enzyme-like catalytic properties. They are attractive reagents because they do not have the same limitations of natural enzymes (e.g., high cost, low stability and difficult storage). To test, optimize and compare nanozymes, it is important to establish fundamental principles and systematic standards to fully characterize their catalytic performance. Our 2018 protocol describes how to characterize the catalytic activity and kinetics of peroxidase nanozymes, the most widely used type of nanozyme. This approach was based on Michaelis-Menten enzyme kinetics and is now updated to take into account the unique physicochemical properties of nanomaterials that determine the catalytic kinetics of nanozymes. The updated procedure describes how to determine the number of active sites as well as other physicochemical properties such as surface area, shape and size. It also outlines how to calculate the hydroxyl adsorption energy from the crystal structure using the density functional theory method. The calculations now incorporate these measurements and computations to better characterize the catalytic kinetics of peroxidase nanozymes that have different shapes, sizes and compositions. This updated protocol better describes the catalytic performance of nanozymes and benefits the development of nanozyme research since further nanozyme development requires precise control of activity by engineering the electronic, geometric structure and atomic configuration of the catalytic sites of nanozymes. The characterization of the catalytic activity of peroxidase nanozymes and the evaluation of their kinetics can be performed in 4 h. The procedure is suitable for users with expertise in nano- and materials technology.
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