Evidence map›Paper›PMID 39147972›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Flagella and Cell Body Staining of Bacteria with Fluorescent Dyes.

Veronika Pfeifer, Valeriia Muraveva, Carsten Beta

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Veronika PfeiferInstitute of Physics and Astronomy, University of Potsdam, Potsdam, Germany.
Valeriia MuravevaInstitute of Physics and Astronomy, University of Potsdam, Potsdam, Germany.
Carsten BetaInstitute of Physics and Astronomy, University of Potsdam, Potsdam, Germany. beta@uni-potsdam.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacteria can propel themselves by rotating a flagellum or a flagellar bundle. To image this thin structure in motile bacteria, the flagella can be vitally stained with fluorophores. This chapter describes a flagellar staining protocol with the additional possibility of visualizing the cell body. It offers the opportunity to track conformational changes of flagella and simultaneously track the positions of the cell bodies. The additional use of a filter increases the number of motile cells and improves the signal-to-noise ratio of images. The flagellar staining requires a prior introduction of a surface-exposed cysteine, which is not covered in this chapter.

Indexed as

BacteriaFlagellaFluorescent DyesStaining and LabelingMicroscopy, FluorescenceFluorescent DyesBacteriaBacteria trackingFlagella-mediated motilityFlagellar configurationFlagellar stainingFlagellumFluorescence imaging

Identifiers

PMID39147972

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.