Evidence map›Paper›PMID 39147836›Full record

ArticleCommunications chemistry2024

Quantitative analysis of electroporation-mediated intracellular delivery via bioorthogonal luminescent reaction.

Shiqi Wang, Mariia V Shcherbii, Sami-Pekka Hirvonen, Gudrun Silvennoinen, Mirkka Sarparanta, Hélder A Santos

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Shiqi WangDrug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00014, Helsinki, Finland. shiqi.wang@helsinki.fi.ORCID http://orcid.org/0000-0003-2010-0132
Mariia V ShcherbiiInstitute of Biotechnology, University of Helsinki, FI-00014, Helsinki, Finland.
Sami-Pekka HirvonenDepartment of Chemistry, Faculty of Science, University of Helsinki, FI-00014, Helsinki, Finland.
Gudrun SilvennoinenDepartment of Chemistry, Faculty of Science, University of Helsinki, FI-00014, Helsinki, Finland.
Mirkka SarparantaDepartment of Chemistry, Faculty of Science, University of Helsinki, FI-00014, Helsinki, Finland.
Hélder A SantosDrug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00014, Helsinki, Finland.

Funding

Academy of Finland (Suomen Akatemia) 318422Academy of Finland (Suomen Akatemia) 331106Academy of Finland (Suomen Akatemia) 331151Academy of Finland (Suomen Akatemia) 346122Academy of Finland (Suomen Akatemia) 354421EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council) BioLure, 101115752European Research Council 101115752
6 · The paper itself

Abstract

Efficient intracellular delivery is crucial for biotherapeutics, such as proteins, oligonucleotides, and CRISPR/Cas9 gene-editing systems, to achieve their efficacy. Despite the great efforts of developing new intracellular delivery carriers, the lack of straightforward methods for intracellular delivery quantification limits further development in this area. Herein, we designed a simple and versatile bioorthogonal luminescent reaction (BioLure assay) to analyze intracellular delivery. Our results suggest that BioLure can be used to estimate the amount of intracellularly delivered molecules after electroporation, and the estimation by BioLure is in good correlation with the results from complementary methods. Furthermore, we used BioLure assay to correlate the intracellularly-delivered RNase A amount with its tumoricidal activity. Overall, BioLure is a versatile tool for understanding the intracellular delivery process on live cells, and establishing the link between the cytosolic concentration of intracellularly-delivered biotherapeutics and their therapeutic efficacy.

Identifiers

PMID39147836
PMCPMC11327378

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.