ArticleBiosensors & bioelectronics2024
Dynamic and large field of view photonic resonator absorption microscopy for ultrasensitive digital resolution detection of nucleic acid and protein biomarkers.
Article in Biosensors & bioelectronics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Photonic resonator absorption microscopy: why consider metallic and magneto-plasmonic nano-assemblies over bare nanoparticles for digital biosensing?Analytical and bioanalytical chemistry · 2025Article
- Oligonucleotide surface attachment by tosylation for digital detection of microRNA using photonic resonator absorption microscopy.APL bioengineering · 2025Article
- Ultrasensitive Alzheimer's disease biomarker detection with nanopillar photonic crystal biosensors.Optica · 2025Article
- Laser-emission vibrational microscopy of microdroplet arrays for high-throughput screening of hyperlipidemia.Light, science & applications · 2025Article
- Physically grounded deep learning-enabled gold nanoparticle localization and quantification in photonic resonator absorption microscopy for digital resolution molecular diagnostics.Biosensors & bioelectronics · 2025Article
- Digital Immunoassay for Rapid Detection of SARS-CoV-2 exposure in a Broad Spectrum of Animals.IEEE sensors journal · 2025Article
- Photonic Crystal Enhanced Microscopy on a 2D Photonic Crystal Surface.Advanced materials technologies · 2025Article
- Portable, smartphone-linked, and miniaturized photonic resonator absorption microscope (PRAM Mini) for point-of-care diagnostics.Biomedical optics express · 2024Article
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Authors and funding
9 authors.
Funding
Abstract
In this paper, we describe a biosensing instrument based on our previously developed photonic resonator absorption microscope (PRAM) that incorporates autofocus, digital representation of the gold nanoparticle (AuNP) accumulation, and the ability to gather time-series image sequences of AuNP attachment and detachment from the photonic crystal (PC) surface. The combined capabilities are used to fully automate PRAM image collection during biomolecular assays to enable tiling of PRAM images to provide millimeter-scale field of view. The instrument can also gather PRAM "movies" that enables digital showcasing and dynamic counting AuNPs as they arrive and depart from the PC surface. We utilize the capabilities in the context of two biomolecular assays for detection of protein biomarkers in a conventional AuNP-tagged sandwich format. Utilizing dynamic counting of AuNP attachment and detachment events during the assay we present a detection for microRNA-375 (miRNA-375) down to 1 aM with a 10-min, room temperature, enzyme-free approach, while revealing characteristics of the binding-rate and unbinding-rate of the biomolecular interactions. Our instrument can potentially find broad applications in multiplexed point-of-care diagnostic testing, and as a general-purpose tool for quantitative characterization of biomolecular binding kinetics with single-molecule resolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.