ArticleScience advances2024
Origin, structure, and composition of the spider major ampullate silk fiber revealed by genomics, proteomics, and single-cell and spatial transcriptomics.
Article in Science advances, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Diverging Liquid-Liquid Phase Separation Behavior of Different Recombinant Major Ampullate Spidroins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Liquid-liquid phase separation enables chromatography-free purification and high-performance spidroin-amyloid hybrid silk fibers.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Cryo-ET comparison of the hierarchical ultrastructure of silkworm, spider, and artificial silk fibers.Nature communications · 2026Article
- An ancient genome duplication event drives the development and evolution of spinnerets in spiders.Science advances · 2026Article
- From candidate genes to omics: Unbiased approaches reshaping arthropod Evo-Devo.Genetics and molecular biology · 2026Article
- Wrinkling-Based Patterning and Recombinant Spider Silk-Based Coating Technologies - Toward Novel Applications.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Spider silk-based structural proteins as tough, biodegradable, and sustainable polymers.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2026Review
- Nanostructured Protein Surfaces Inspired by Spider Silk.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- On-the-Fly Synthesis of Freestanding Spin-Crossover Architectures With Tunable Magnetic Properties.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Native Silk Fibers: Protein Sequence and Structure Influences on Thermal and Mechanical Properties.Biomacromolecules · 2025Review
- The Biology of Natural Polymers Accelerates and Expands the Science of Biomacromolecules: A Focus on Structural Proteins.Biomacromolecules · 2025Review
- Silk Protein Gene Engineering and Its Applications: Recent Advances in Biomedicine Driven by Molecular Biotechnology.Drug design, development and therapy · 2025Review
- Effects of Mini-Spidroin Repeat Region on the Mechanical Properties of Artificial Spider Silk Fibers.ACS omega · 2024Article
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
Spiders produce nature's toughest fiber using renewable components at ambient temperatures and with water as solvent, making it highly interesting to replicate for the materials industry. Despite this, much remains to be understood about the bioprocessing and composition of spider silk fibers. Here, we identify 18 proteins that make up the spiders' strongest silk type, the major ampullate fiber. Single-cell RNA sequencing and spatial transcriptomics revealed that the secretory epithelium of the gland harbors six cell types. These cell types are confined to three distinct glandular zones that produce specific combinations of silk proteins. Image analysis of histological sections showed that the secretions from the three zones do not mix, and proteomics analysis revealed that these secretions form layers in the final fiber. Using a multi-omics approach, we provide substantial advancements in the understanding of the structure and function of the major ampullate silk gland as well as of the architecture and composition of the fiber it produces.
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Registered trials
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