Evidence map›Paper›PMID 39141519›Full record

ArticleSTAR protocols2024

Protocol for isolating extracellular vesicles from pancreatic cancer cells for liver metastatic niche research.

Anthony Casabianca, Mélanie Bellina, Crissy Dudgeon, Muhammad Sadeqi Nezhad, Chris Harris, Stephany Fiore, Agnes Bernet, Wade Narrow, Orjola Prela, Lan Wang and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Anthony CasabiancaDepartment of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA.
Mélanie BellinaApoptosis, Cancer and Development Laboratory - Equipe labellisée 'La Ligue', LabEx DEVweCAN, Institut Convergence PLAsCAN, Centre de Recherche en Cancérologie de Lyon (CRCL), INSERM U1052-CNRS UMR5286, Université de Lyon, Université Claude Bernard Lyon1, Centre Léon Bérard, 69008 Lyon, France; Netris Pharma, 69008 Lyon. France.
Crissy DudgeonRutgers Cancer Institute of New Jersey, New Brunswick, NJ 08901, USA.
Muhammad Sadeqi NezhadUniversity of Rochester Clinical and Translational Science Institute, Rochester, NY 14620, USA.
Chris HarrisDepartment of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA; Wilmot Cancer Center, University of Rochester, Rochester, NY 14620, USA.
Stephany FioreNetris Pharma, 69008 Lyon. France.
Agnes BernetApoptosis, Cancer and Development Laboratory - Equipe labellisée 'La Ligue', LabEx DEVweCAN, Institut Convergence PLAsCAN, Centre de Recherche en Cancérologie de Lyon (CRCL), INSERM U1052-CNRS UMR5286, Université de Lyon, Université Claude Bernard Lyon1, Centre Léon Bérard, 69008 Lyon, France; Netris Pharma, 69008 Lyon. France.
Wade NarrowDepartment of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA; Wilmot Cancer Center, University of Rochester, Rochester, NY 14620, USA.
Orjola PrelaDepartment of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA.
Lan WangDepartment of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA.
Igor AstsaturovDepartment of Medical Oncology, Fox Chase Cancer Center, Philadephia, PA 19111, USA.
Patrick MehlenApoptosis, Cancer and Development Laboratory - Equipe labellisée 'La Ligue', LabEx DEVweCAN, Institut Convergence PLAsCAN, Centre de Recherche en Cancérologie de Lyon (CRCL), INSERM U1052-CNRS UMR5286, Université de Lyon, Université Claude Bernard Lyon1, Centre Léon Bérard, 69008 Lyon, France; Netris Pharma, 69008 Lyon. France.
Darren R CarpizoDepartment of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA; Wilmot Cancer Center, University of Rochester, Rochester, NY 14620, USA. Electronic address: darren_carpizo@urmc.rochester.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular vesicles (EVs) are secreted, cell-derived, membrane-bound compartments implicated in various diseases for their ability to influence distant targets and as carriers of biomarkers. Here, we present a protocol for separating EVs from mammalian pancreatic cancer cells and their characterization using western blot and electron microscopy. We then demonstrate how they are utilized to affect tumor development in a murine model of metastatic pancreatic cancer including a method to quantify hepatic tumor burden in histologic samples. For complete details on the use and execution of this protocol, please refer to Dudgeon et al.

Indexed as

Extracellular VesiclesLiver NeoplasmsPancreatic NeoplasmsAnimalsCell Line, TumorHumansMiceCancerCell BiologyMolecular Biology

Identifiers

PMID39141519
PMCPMC11519706

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.