Evidence map›Paper›PMID 39141108›Full record

ArticleMedical molecular morphology2024

Molecular and ultrastructural morphological analyses of highly metamorphosed Aspergillus fumigatus on human formalin-fixed paraffin-embedded tissue.

Kazuhiro Matsumoto, Masanori Goto, Yuki Kamikokura, Kumi Takasawa, Nobuyuki Kobayashi, Tomoyuki Aoyama, Taro Murakami, Masayo Kamikokura, Yuta Ikechi, Tomoki Kawahata and 7 more

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In one paragraph

Article in Medical molecular morphology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Kazuhiro MatsumotoDivision of Tumor Pathology, Department of Pathology, Asahikawa Medical University School of Medicine, Higashi 2-1-1-1, Midorigaoka, Asahikawa, 078-8510, Japan.
Masanori GotoDivision of Tumor Pathology, Department of Pathology, Asahikawa Medical University School of Medicine, Higashi 2-1-1-1, Midorigaoka, Asahikawa, 078-8510, Japan. gotom@asahikawa-med.ac.jp.ORCID http://orcid.org/0000-0003-1760-5252
Yuki KamikokuraDivision of Tumor Pathology, Department of Pathology, Asahikawa Medical University School of Medicine, Higashi 2-1-1-1, Midorigaoka, Asahikawa, 078-8510, Japan.
Kumi TakasawaDivision of Tumor Pathology, Department of Pathology, Asahikawa Medical University School of Medicine, Higashi 2-1-1-1, Midorigaoka, Asahikawa, 078-8510, Japan.
Nobuyuki KobayashiMedical Laboratory and Blood Center, Asahikawa Medical University Hospital, Asahikawa, Japan.
Tomoyuki AoyamaDepartment of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
Taro MurakamiDepartment of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
Masayo KamikokuraDivision of Tumor Pathology, Department of Pathology, Asahikawa Medical University School of Medicine, Higashi 2-1-1-1, Midorigaoka, Asahikawa, 078-8510, Japan.
Yuta IkechiDivision of Endocrinology, Metabolism, and Rheumatology Department of Internal Medicine, Asahikawa Medical University Hospital, Asahikawa, Japan.
Tomoki KawahataDivision of Endocrinology, Metabolism, and Rheumatology Department of Internal Medicine, Asahikawa Medical University Hospital, Asahikawa, Japan.
Kitaru TanakaDivision of Endocrinology, Metabolism, and Rheumatology Department of Internal Medicine, Asahikawa Medical University Hospital, Asahikawa, Japan.
Sayaka TakatoriDivision of Endocrinology, Metabolism, and Rheumatology Department of Internal Medicine, Asahikawa Medical University Hospital, Asahikawa, Japan.
Daisuke FujishiroDivision of Endocrinology, Metabolism, and Rheumatology Department of Internal Medicine, Asahikawa Medical University Hospital, Asahikawa, Japan.
Kensaku OkamotoDivision of Endocrinology, Metabolism, and Rheumatology Department of Internal Medicine, Asahikawa Medical University Hospital, Asahikawa, Japan.
Yuichi MakinoCenter for Integrated Medical Education and Regional Symbiosis, Asahikawa Medical University Hospital, Asahikawa, Japan.
Yuji NishikawaAsahikawa Medical University Hospital, Asahikawa, Japan.
Akira TakasawaDivision of Tumor Pathology, Department of Pathology, Asahikawa Medical University School of Medicine, Higashi 2-1-1-1, Midorigaoka, Asahikawa, 078-8510, Japan. atakasawa@asahikawa-med.ac.jp.

Funding

Japan Society for the Promotion of Science JP22K06944
6 · The paper itself

Abstract

Invasive fungal infections including invasive pulmonary aspergillosis (IPA) generally have a poor prognosis, because the fungi spread throughout various organs. Therefore, it is important to accurately identify the fungal species for treatment. In this article, we present the results of pathological and molecular morphological analyses that were performed to elucidate the cause of respiratory failure in a patient who died despite suspicion of IPA and treatment with micafungin (MCFG). Pathological analysis revealed the existence of cystic and linear fungi in lung tissue. The fungi were identified as Aspergillus fumigatus (A. fumigatus) by partial sequencing of genomic DNA. Correlative light microscopy and electron microscopy (CLEM) analysis confirmed that fungi observed with light microscopy can also be observed with scanning electron microscopy (SEM) using formalin-fixed paraffin-embedded tissue sections. SEM revealed an atypical ultrastructure of the fungi including inhomogeneous widths, rough surfaces, and numerous cyst-like structures of various sizes. The fungi showed several morphological changes of cultured A. fumigatus treated with MCFG that were previously reported. Our results indicate that integrated analysis of ultrastructural observation by SEM and DNA sequencing may be an effective tool for analyzing fungi that are difficult to identify by conventional pathological analysis.

Indexed as

Aspergillus fumigatusMicroscopy, Electron, ScanningParaffin EmbeddingDNA, FungalEchinocandinsFormaldehydeHumansInvasive Pulmonary AspergillosisLungMaleMicafunginSequence Analysis, DNATissue FixationDNA, FungalEchinocandinsFormaldehydeMicafunginAspergillusDNA sequencingFormalin-fixed paraffin-embedded tissueInvasive pulmonary aspergillosisMicafunginMorphological changesScanning Electron Microscope

Identifiers

PMID39141108

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.