Evidence map›Paper›PMID 39140467›Full record

ArticleJournal of extracellular vesicles2024

Comparison of EV characterization by commercial high-sensitivity flow cytometers and a custom single-molecule flow cytometer.

James Kim, Shihan Xu, Seung-Ryoung Jung, Alya Nguyen, Yuanhua Cheng, Mengxia Zhao, Bryant S Fujimoto, Wyatt Nelson, Perry Schiro, Jeffrey L Franklin and 21 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

31 authors.

James KimDepartment of Chemistry, University of Washington, Seattle, Washington, USA.ORCID https://orcid.org/0000-0002-6990-509X
Shihan XuDepartment of Chemistry, University of Washington, Seattle, Washington, USA.
Seung-Ryoung JungDepartment of Chemistry, University of Washington, Seattle, Washington, USA.
Alya NguyenDepartment of Chemistry, University of Washington, Seattle, Washington, USA.
Yuanhua ChengDepartment of Chemistry, University of Washington, Seattle, Washington, USA.
Mengxia ZhaoDepartment of Chemistry, University of Washington, Seattle, Washington, USA.
Bryant S FujimotoDepartment of Chemistry, University of Washington, Seattle, Washington, USA.
Wyatt NelsonDepartment of Chemistry, University of Washington, Seattle, Washington, USA.
Perry SchiroMicareo Inc, Burlingame, California, USA.
Jeffrey L FranklinDepartment of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA.
James N HigginbothamDepartment of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA.
Robert J CoffeyDepartment of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA.ORCID https://orcid.org/0000-0002-2180-3844
Min ShiDepartment of Pathology, University of Washington, Seattle, Washington, USA.
Lucia N VojtechDepartment of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA.
Florian HladikDepartment of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA.
Muneesh TewariDivision of Hematology/Oncology, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA.
John TiggesDepartment of Medicine, Beth Israel Deaconess Medical Center, Boston and Cambridge, Massachusetts, USA.
Ionita GhiranDepartment of Medicine, Beth Israel Deaconess Medical Center, Boston and Cambridge, Massachusetts, USA.ORCID https://orcid.org/0000-0001-6655-3298
Tijana Jovanovic-TalismanDepartment of Molecular Medicine, Beckman Research Institute of the City of Hope Comprehensive Cancer Center, Duarte, California, USA.
Louise C LaurentDepartment of Obstetrics, Gynecology, and Reproductive Sciences and Sanford Consortium for Regenerative Medicine, University of California San Diego, San Diego, California, USA.
Saumya DasCardiovascular Research Center, Massachusetts General Hospital, Harvard Medical school, Boston, Massachusetts, USA.
Olesia GololobovaDepartment of Molecular and Comparative Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.ORCID https://orcid.org/0000-0002-0392-268X
Kenneth W WitwerDepartment of Molecular and Comparative Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.ORCID https://orcid.org/0000-0003-1664-4233
Tuoye XuCancer Research Institute, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.
Al CharestCancer Research Institute, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.
Kendall Van Keuren JensenNeurogenomics Division, TGen, Phoenix, Arizona, USA.
Robert L RaffaiDepartment of Veterans Affairs, Surgical Service (112G), San Francisco VA Medical Center, San Francisco, California, USA.ORCID https://orcid.org/0000-0002-5442-3055
Jennifer C JonesTranslational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
Joshua A WelshTranslational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.ORCID https://orcid.org/0000-0002-1097-9756
John P NolanScintillon Institute, San Diego, California, USA.
Daniel T ChiuDepartment of Chemistry, University of Washington, Seattle, Washington, USA.

Funding

University of Washington/Fred Hutch Center for AIDS ResearchP30AI027757 · NIAID · UNIVERSITY OF WASHINGTON · PI CONNIE L CELUM · 1988 to 2026
$104.9M
Understanding and Controlling p120 Dysfunction in CRCP50CA095103 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI COFFEY, ROBERT J. · 2002 to 2017
$33.6M
Translational Analysis CoreP30DK058404 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI MARY Kay WASHINGTON · 2002 to 2026
$29.9M
Vanderbilt-Ingram Cancer Center SPORE in Gastrointestinal CancerP50CA236733 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI STEPHEN W. FESIK · 2019 to 2026
$19.6M
Roles for Supermeres in CRC ProgressionP01CA229123 · NCI · VANDERBILT UNIVERSITY · PI Alissa M Weaver · 2020 to 2026
$12.9M
Integrated approach to study early and late events in colonic neoplasia: mouse to manR35CA197570 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Robert J. Coffey · 2017 to 2026
$9.4M
MicroRNAs in CNS-derived extracellular microvesicles as peripheral blood biomarkers for Alzheimer diseaseR01AG061383 · NIA · UNIVERSITY OF WASHINGTON · PI SHI, MIN · 2019 to 2023
$3.4M
Extracellular vesicle transport of brain-derived proteins to the blood in Alzheimer diseaseRF1AG068406 · NIA · UNIVERSITY OF WASHINGTON · PI SHI, MIN · 2020 to 2020
$3.0M
Molecular dissection and imaging of extracellular vesicles to define their origin and targetsUH3TR002878 · NCATS · MASSACHUSETTS GENERAL HOSPITAL · PI DAS, SAUMYA, TALISMAN, TIJANA · 2021 to 2022
$2.2M
Uncovering exRNA and protein determinants of secreted vesicle heterogeneity by flow cytometric purification of vesicle subsets from cells and plasmaUH3CA241685 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI CHAREST, ALAIN, COFFEY, ROBERT J. · 2021 to 2022
$2.1M
Characterization and quantification of CNS cell specific extracellular microvesicles in bloodR33MH118160 · NIMH · UNIVERSITY OF WASHINGTON · PI SHI, MIN · 2020 to 2022
$2.0M
Single Extracellular Vesicle Sorting and AnalysisUH3TR002874 · NCATS · UNIVERSITY OF WASHINGTON · PI CHIU, DANIEL T · 2021 to 2022
$2.0M
BLRD VA I01 BX003928BLRD VA IK6 BX005692National Institute of HealthNCATS NIH HHS UG3 TR002878NCATS NIH HHS UH3 TR002874NCATS NIH HHS UH3 TR002878NCI NIH HHS P01 CA229123NCI NIH HHS P50 CA236733NCI NIH HHS R35 CA197570NCI NIH HHS UG3 CA241685NCI NIH HHS UH3 CA241685NCI NIH HHS UH3 CA241703NIAID NIH HHS P30 AI027757NIA NIH HHS R01 AG061383NIA NIH HHS R01 AG068406NIA NIH HHS R21 AG086722NIA NIH HHS RF1 AG068406NIDDK NIH HHS P30 DK058404NIMH NIH HHS R33 MH118160VA Merit grant
6 · The paper itself

Abstract

High-sensitivity flow cytometers have been developed for multi-parameter characterization of single extracellular vesicles (EVs), but performance varies among instruments and calibration methods. Here we compare the characterization of identical (split) EV samples derived from human colorectal cancer (DiFi) cells by three high-sensitivity flow cytometers, two commercial instruments, CytoFLEX/CellStream, and a custom single-molecule flow cytometer (SMFC). DiFi EVs were stained with the membrane dye di-8-ANEPPS and with PE-conjugated anti-EGFR or anti-tetraspanin (CD9/CD63/CD81) antibodies for estimation of EV size and surface protein copy numbers. The limits of detection (LODs) for immunofluorescence and vesicle size based on calibration using cross-calibrated, hard-dyed beads were ∼10 PE/∼80 nm EV diameter for CytoFLEX and ∼10 PEs/∼67 nm for CellStream. For the SMFC, the LOD for immunofluorescence was 1 PE and ≤ 35 nm for size. The population of EVs detected by each system (di-8-ANEPPS

Indexed as

Extracellular VesiclesFlow CytometryCell Line, TumorColorectal NeoplasmsHumansSingle Molecule ImagingCellStreamCytoFLEXequivalent reference fluorophore calibration beadsextracellular vesicleslimit of detectionsingle‐molecule flow cytometry

Identifiers

PMID39140467
PMCPMC11322860

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.