ArticleNeurophotonics2024
Optical constraints on two-photon voltage imaging.
Article in Neurophotonics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed.
- Methods, Trade-offs, and Opportunities in High-speed Optical Microscopy for Neural Voltage imaging.Nature photonics · 2026Article
- Voltage imaging of neurons distributed across entire brains of larval zebrafish.Nature methods · 2026Article
- FACED 2.0 enables large-scale voltage and calcium imaging in vivo.Nature methods · 2026Article
- A versatile platform for two-photon neuronal population voltage imaging across cortical depths.Nature methods · 2026Article
- Article
- Simultaneous In Vivo Electrophysiology, Two-Photon Imaging, and Optogenetics for Probing Neurovascular Coupling.Methods and protocols · 2026Article
- A multimodal approach for visualizing and identifying electrophysiological cell types in vivo.Nature communications · 2026Article
- A versatile, positive-going voltage indicator that enables accessible two-photon recordings in vivo.bioRxiv : the preprint server for biology · 2026Article
- Glutamate indicators with increased sensitivity and tailored deactivation rates.Nature methods · 2026Article
- Voltage imaging as a window into neural computation.Neurophotonics · 2026Article
- Article
- Voltage imaging with periodic structured illumination.Biomedical optics express · 2026Article
- Miniaturized widefield microscope for high speed in vivo voltage imaging.Biomedical optics express · 2026Article
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Miniaturized widefield microscope for high speed in vivo voltage imaging.bioRxiv : the preprint server for biology · 2025Article
- Voltage Imaging with Periodic Structured Illumination.bioRxiv : the preprint server for biology · 2025Article
- Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators.Science advances · 2025Article
- Deep two-photon voltage imaging with adaptive excitation.Research square · 2024Article
- Scanless two-photon voltage imaging.Nature communications · 2024Article
- Updated Toolbox for Assessing Neuronal Network Reconstruction after Cell Therapy.Bioengineering (Basel, Switzerland) · 2024Review
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4 authors.
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Abstract
Significance: Genetically encoded voltage indicators (GEVIs) are a valuable tool for studying neural circuits Aim: We consider the optical and biophysical constraints particular to 1P and 2P voltage imaging and compare the imaging properties of commonly used GEVIs under 1P and 2P excitation. Approach: We measure the brightness and voltage sensitivity of voltage indicators from commonly used classes under 1P and 2P illumination. We also measure the decrease in fluorescence as a function of depth in the mouse brain. We develop a simple model of the number of measurable cells as a function of reporter properties, imaging parameters, and desired signal-to-noise ratio (SNR). We then discuss how the performance of voltage imaging would be affected by sensor improvements and by recently introduced advanced imaging modalities. Results: Compared with 1P excitation, 2P excitation requires Conclusions: Due to the stringent photon-count requirements of voltage imaging and the modest voltage sensitivity of existing reporters, 2P voltage imaging
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