Evidence map›Paper›PMID 39139574›Full record

ArticleFrontiers in immunology2024

Circ_BBS9 as an early diagnostic biomarker for lung adenocarcinoma: direct interaction with IFIT3 in the modulation of tumor immune microenvironment.

Daijun Peng, Mingyu Liang, Lingyu Li, Haisheng Yang, Di Fang, Lingling Chen, Bing Guan

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Article in Frontiers in immunology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Daijun PengDepartment of Pathology, Jinshan Branch of Shanghai Sixth People's Hospital, Shanghai, China.
Mingyu LiangDepartment of Automation, Shanghai Jiao Tong University, Shanghai, China.
Lingyu LiDepartment of Pathology, Jinshan Branch of Shanghai Sixth People's Hospital, Shanghai, China.
Haisheng YangDepartment of Pathology, Jinshan Branch of Shanghai Sixth People's Hospital, Shanghai, China.
Di FangDepartment of Pathology, Jinshan Branch of Shanghai Sixth People's Hospital, Shanghai, China.
Lingling ChenDepartment of Pathology, Jinshan Branch of Shanghai Sixth People's Hospital, Shanghai, China.
Bing GuanDepartment of Pathology, Jinshan Branch of Shanghai Sixth People's Hospital, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Introduction: Circular RNAs (circRNAs) have been identified as significant contributors to the development and advancement of cancer. The objective of this study was to examine the expression and clinical implications of circRNA circ_BBS9 in lung adenocarcinoma (LUAD), as well as its potential modes of action. Methods: The expression of Circ_BBS9 was examined in tissues and cell lines of LUAD through the utilization of microarray profiling, quantitative real-time polymerase chain reaction (qRT-PCR), and western blot analysis. In this study, we assessed the impact of circ_BBS9 on the proliferation of LUAD cells, as well as its influence on ferroptosis and tumor formation. To analyze these effects, we employed CCK-8 assays and ferroptosis assays. The identification of proteins that interact with Circ_BBS9 was achieved through the utilization of RNA pull-down and mass spectrometry techniques. A putative regulatory network comprising circ_BBS9, miR-7150, and IFIT3 was established using bioinformatics study. The investigation also encompassed the examination of the correlation between the expression of IFIT3 and the invasion of immune cells. Results: Circ_BBS9 was significantly downregulated in LUAD tissues and cell lines. Low circ_BBS9 expression correlated with poor prognosis. Functional experiments showed that circ_BBS9 overexpression inhibited LUAD cell proliferation and promoted ferroptosis Conclusion: Circ_BBS9 has been identified as a tumor suppressor in lung adenocarcinoma (LUAD) and holds promise as a diagnostic biomarker. The potential mechanism of action involves the modulation of ferroptosis and the immunological microenvironment through direct interaction with IFIT3 and competitive binding to miR-7150. The aforementioned findings offer new perspectives on the pathophysiology of LUAD and highlight circ_BBS9 as a potentially valuable target for therapeutic interventions.

Indexed as

Adenocarcinoma of LungBiomarkers, TumorFerroptosisGene Expression Regulation, NeoplasticLung NeoplasmsRNA, CircularTumor MicroenvironmentAnimalsApoptosis Regulatory ProteinsCell Line, TumorCell ProliferationFemaleHumansIntracellular Signaling Peptides and ProteinsMaleMiceApoptosis Regulatory ProteinsBiomarkers, TumorIFIT3 protein, humanIntracellular Signaling Peptides and ProteinsMicroRNAsRNA-Binding ProteinsRNA, Circularcirc_BBS9circular ribonucleic acids (circRNAs)ferroptosisIFIT3immune microenvironmentlung adenocarcinoma (LUAD)

Identifiers

PMID39139574
PMCPMC11320841

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.