ArticlePlant communications2024
CRISPR-Cas9-mediated construction of a cotton CDPK mutant library for identification of insect-resistance genes.
Article in Plant communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Novel effector HYPB1 of cotton bollworm (Helicoverpa armigera) inhibits biosynthesis of plant secondary metabolites and promotes feeding by targeting cotton dirigent protein GhDIR15.Journal of integrative plant biology · 2026Article
- Three Decades of China's Bt Cotton: Achievements and Insights.Plant biotechnology journal · 2026Review
- Overexpression of GhAAP3 increases Helicoverpa armigera feeding by altering hormonal and metabolic composition in cotton.Plant cell reports · 2026Article
- Accelerating Resistance Breeding: Emerging Methods to Identify and Validate Plant Immunity Genes.Plants (Basel, Switzerland) · 2026Review
- Multiplex Gene Editing Creates Triple-Resistant Rice Against Both Insect Herbivores and Pathogens.Plants (Basel, Switzerland) · 2026Article
- Breeding jassid-resistant okra (Frontiers in plant science · 2026Review
- Large-Scale Rice Mutant Establishment and High-Throughput Mutant Manipulation Help Advance Rice Functional Genomics.Plants (Basel, Switzerland) · 2025Review
- Construction of multi-targeted CRISPR libraries in tomato to overcome functional redundancy at genome-scale level.Nature communications · 2025Article
- Calcium-dependent protein kinases as central hubs in plant abiotic stress signaling: mechanisms and prospects for crop improvement.Frontiers in plant science · 2025Review
- Article
- CRISPR/Cas9-based mutant library screening: the discovery of novel genes regulating immune responses in cotton and rice.Frontiers in plant science · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors.
Funding
Abstract
Calcium-dependent protein kinases (CDPKs) act as key signal transduction enzymes in plants, especially in response to diverse stresses, including herbivory. In this study, a comprehensive analysis of the CDPK gene family in upland cotton revealed that GhCPKs are widely expressed in multiple cotton tissues and respond positively to various biotic and abiotic stresses. We developed a strategy for screening insect-resistance genes from a CRISPR-Cas9 mutant library of GhCPKs. The library was created using 246 single-guide RNAs targeting the GhCPK gene family to generate 518 independent T0 plants. The average target-gene coverage was 86.18%, the genome editing rate was 89.49%, and the editing heritability was 82%. An insect bioassay in the field led to identification of 14 GhCPK mutants that are resistant or susceptible to insects. The mutant that showed the clearest insect resistance, cpk33/74 (in which the homologous genes GhCPK33 and GhCPK74 were knocked out), was selected for further study. Oral secretions from Spodoptera litura induced a rapid influx of Ca
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.