Evidence map›Paper›PMID 39136491›Full record

ArticleApplied and environmental microbiology2024

Unraveling the genetic potential of nitrous oxide reduction in wastewater treatment: insights from metagenome-assembled genomes.

Patrick Skov Schacksen, Jeppe Lund Nielsen

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Patrick Skov SchacksenDepartment of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.ORCID 0000-0002-3850-4971
Jeppe Lund NielsenDepartment of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.ORCID 0000-0002-8747-6938

Funding

Teknologi og Produktion, Det Frie Forskningsråd (FTP, DFF) 9041-00367B
6 · The paper itself

Abstract

This study explores the genetic landscape of nitrous oxide (N IMPORTANCE: This study provides critical insights into the genetic diversity of nitrous oxide reductase (nosZ) genes and the microorganisms harboring them in wastewater treatment plants (WWTPs) by exploring 1,083 high-quality metagenome-assembled genomes (MAGs) from 23 Danish full-scale WWTPs. Despite the pivotal role of nosZ-containing organisms, their diversity remains largely unexplored in WWTPs. Our custom pipeline for detecting nosZ provides near-full-length genes with detailed information on secretory pathways and accessory nos genes. Using these genes as templates, we developed taxonomically diverse clade-specific primers that generate nosZ amplicons for phylogenetic annotation and gene-to-MAG linkage. This approach improves detection and expands the discovery of novel sequences, highlighting the prevalence of non-denitrifying N

Indexed as

MetagenomeNitrous OxideWastewaterBacteriaDenmarkGenome, BacterialOxidation-ReductionOxidoreductasesPhylogenyWaste Disposal, FluidNitrous Oxidenitrous oxide reductaseOxidoreductasesWastewaternitrogen metabolismnitrous oxide reductasenitrous oxide sinkNosZwastewater treatment

Identifiers

PMID39136491
PMCPMC11409646

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.