Evidence map›Paper›PMID 39135241›Full record

ArticleThe Journal of reproduction and development2024

Three-dimensional cell culture using CD9-positive cells isolated from marginal cell layer of intermediate lobe of rats sustains in vivo-like primary niche environment.

Kotaro Horiguchi, Takehiro Tsukada, Saishu Yoshida, Ken Fujiwara, Takashi Nakakura, Morio Azuma, Ayano Shindo, Rumi Hasegawa, Shu Takigami

Abstract read
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In one paragraph

Article in The Journal of reproduction and development, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Kotaro HoriguchiLaboratory of Anatomy and Cell Biology, Department of Health Sciences, Kyorin University, Tokyo 181-8612, Japan.
Takehiro TsukadaDepartment of Biomolecular Science, Faculty of Science, Toho University, Chiba 274-8510, Japan.
Saishu YoshidaDepartment of Biomolecular Science, Faculty of Science, Toho University, Chiba 274-8510, Japan.
Ken FujiwaraDepartment of Biological Science, Faculty of Science, Kanagawa University, Kanagawa 259-1293, Japan.
Takashi NakakuraDepartment of Anatomy, Graduate School of Medicine, Teikyo University, Tokyo 173-8605, Japan.
Morio AzumaDepartment of Pharmacology, Graduate School of Medicine, Jichi Medical University, Tochigi 329-0498, Japan.
Ayano ShindoLaboratory of Anatomy and Cell Biology, Department of Health Sciences, Kyorin University, Tokyo 181-8612, Japan.
Rumi HasegawaLaboratory of Anatomy and Cell Biology, Department of Health Sciences, Kyorin University, Tokyo 181-8612, Japan.
Shu TakigamiLaboratory of Anatomy and Cell Biology, Department of Health Sciences, Kyorin University, Tokyo 181-8612, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The adenohypophysis is composed of the anterior and intermediate lobes (AL and IL, respectively), and secretes hormones that play an important role in reproduction. CD9- and SOX2-double (CD9/SOX2) positive cells located in the marginal cell layer (MCL) facing the Rathke's cleft in the AL and IL form the primary stem cell niche in the adult adenohypophysis of rats. In this study, we successfully obtained 3-dimensional (3D) cell aggregates that closely resembled the primary niche of MCL in vivo. After incubation in a Matrigel containing several growth factors, approximately 20% of the cells in the CD9/SOX2-positive cell aggregates were differentiated into hormone-producing cells. The cell aggregates generated in this study may provide insight into the regulation of the pituitary stem/progenitor cell niche and the turnover of hormone-producing cells.

Indexed as

Cell DifferentiationSOXB1 Transcription FactorsStem Cell NicheTetraspanin 29AnimalsCell Culture Techniques, Three DimensionalCells, CulturedCollagenDrug CombinationsLamininMalePituitary Gland, AnteriorProteoglycansRatsCollagenDrug CombinationsLamininmatrigelProteoglycansSox2 protein, ratSOXB1 Transcription FactorsTetraspanin 29CD9Hanging drop three-dimensional cell cultureIntermediate lobePituitary glandStem cells

Identifiers

PMID39135241
PMCPMC11461522

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.