ArticleProceedings of the National Academy of Sciences of the United States of America2024
An artificially evolved gene for herbicide-resistant rice breeding.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Knockout of OsRbohD (the NADPH Oxidase Gene) Enhances Saline-Alkaline Stress Tolerance and Grain Yield in Rice by Reducing ROS Accumulation.Rice (New York, N.Y.) · 2026Article
- Time-resolved transcriptomic analysis suggests candidate hub gene modules and putative regulatory pathways in tobacco under dicamba stress.Frontiers in plant science · 2026Article
- Comprehensive characterization and diversity analysis of the HIS1 gene family in rice subpopulations for herbicide resistance.BMC plant biology · 2025Article
- RNAi and genome editing of sugarcane: Progress and prospects.The Plant journal : for cell and molecular biology · 2025Review
- Prime editing using paired pegRNAs targeting NG- or NGG-PAM in rice.Frontiers in genome editing · 2025Article
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Authors and funding
11 authors.
Funding
Abstract
Discovering and engineering herbicide-resistant genes is a crucial challenge in crop breeding. This study focuses on the 4-hydroxyphenylpyruvate dioxygenase Inhibitor Sensitive 1-Like (HSL) protein, prevalent in higher plants and exhibiting weak catalytic activity against many β-triketone herbicides (β-THs). The crystal structures of maize HSL1A complexed with β-THs were elucidated, identifying four essential herbicide-binding residues and explaining the weak activity of HSL1A against the herbicides. Utilizing an artificial evolution approach, we developed a series of rice HSL1 mutants targeting the four residues. Then, these mutants were systematically evaluated, identifying the M10 variant as the most effective in modifying β-THs. The initial active conformation of substrate binding in HSL1 was also revealed from these mutants. Furthermore, overexpression of M10 in rice significantly enhanced resistance to β-THs, resulting in a notable 32-fold increase in resistance to methyl-benquitrione. In conclusion, the artificially evolved M10 gene shows great potential for the development of herbicide-resistant crops.
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