ArticleJournal of the American Chemical Society2024
Measuring Integrin Force Loading Rates Using a Two-Step DNA Tension Sensor.
Article in Journal of the American Chemical Society, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Force-Locking DNA Hairpin Probes for High-Throughput and Cumulative Detection of Intercellular Molecular Tensions.Angewandte Chemie (International ed. in English) · 2026Article
- Integrin-mediated mechanotransduction in the tumor microenvironment: macrophage-centered signaling mechanisms and immune remodeling.Journal of translational medicine · 2026Review
- Integrin α2: mode of regulation and functioning in the metastatic cancer cascade.Journal of translational medicine · 2026Review
- Mechanotransduction through T cell receptors: consensus, controversies and future outlooks.Experimental & molecular medicine · 2026Review
- Guidance for 3D traction force microscopy today and in the next decade.Nature methods · 2026Review
- Synthetic mechanoreceptor engineering: From genetic encoding to DNA nanotechnology-based reprogramming.Mechanobiology in medicine · 2025Review
- DNA Motors Powered by Exonuclease III for Autonomous Rolling Motion and Biosensing Applications.bioRxiv : the preprint server for biology · 2025Article
- Capped high-force integrin bond lifetimes and spacing-tuned binding frequency drive rapid fibroblast migration.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The mechanical response of vinculin.Science advances · 2025Article
- Sensitive Detection of Intercellular Tensile Forces via Cas12a-Assisted Membrane Molecular Probes.Nano letters · 2025Article
- DNA Origami Tension Sensors (DOTS) for Single-Molecule Force Measurements at Fluid Intermembrane Junctions.Nano letters · 2025Article
- Decoy DNA Protects Molecular Tension Probes from DNase Degradation.Angewandte Chemie (International ed. in English) · 2025Article
- Quantitative Super-Resolution Imaging of Molecular Tension.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Live-cell imaging of single integrin tensions with minimal background fluorescence noise.Biophysical journal · 2025Article
- Measuring and manipulating mechanical forces during development.Nature cell biology · 2025Review
- Digital and Tunable Genetically Encoded Tension Sensors Based on Engineered Coiled-Coils.Angewandte Chemie (International ed. in English) · 2025Article
- Analytical methods in studying cell force sensing: principles, current technologies and perspectives.Regenerative biomaterials · 2025Review
- Integrin force loading rate in mechanobiology: From model to molecular measurement.QRB discovery · 2025Review
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7 authors.
Funding
Abstract
Cells apply forces to extracellular matrix (ECM) ligands through transmembrane integrin receptors: an interaction which is intimately involved in cell motility, wound healing, cancer invasion and metastasis. These small (piconewton) integrin-ECM forces have been studied by molecular tension fluorescence microscopy (MTFM), which utilizes a force-induced conformational change of a probe to detect mechanical events. MTFM has revealed the force magnitude for integrin receptors in a variety of cell models including primary cells. However, force dynamics and specifically the force loading rate (LR) have important implications in receptor signaling and adhesion formation and remain poorly characterized. Here, we develop an LR probe composed of an engineered DNA structure that undergoes two mechanical transitions at distinct force thresholds: a low force threshold at 4.7 pN (hairpin unfolding) and a high force threshold at 47 pN (duplex shearing). These transitions yield distinct fluorescence signatures observed through single-molecule fluorescence microscopy in live cells. Automated analysis of tens of thousands of events from eight cells showed that the bond lifetime of integrins that engage their ligands and transmit a force >4.7 pN decays exponentially with a τ of 45.6 s. A subset of these events mature in magnitude to >47 pN with a median loading rate of 1.1 pN s
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.