Evidence map›Paper›PMID 39131357›Full record

ArticlebioRxiv : the preprint server for biology2024

Appendage regeneration requires IMPDH2 and creates a sensitized environment for enzyme filament formation.

Morgan E McCartney, Gavin M Wheeler, Audrey G O'Neill, Jeet H Patel, Zoey R Litt, S John Calise, Justin M Kollman, Andrea E Wills

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Morgan E McCartneyDepartment of Biochemistry. University of Washington, Seattle WA.
Gavin M WheelerDepartment of Biochemistry. University of Washington, Seattle WA.
Audrey G O'NeillDepartment of Biochemistry. University of Washington, Seattle WA.ORCID 0000-0003-3964-4236
Jeet H PatelDepartment of Cell and Developmental Biology, University of Pennsylvania, Philadelphia PA.
Zoey R LittDepartment of Biochemistry. University of Washington, Seattle WA.
S John CaliseDepartment of Biochemistry. University of Washington, Seattle WA.
Justin M KollmanDepartment of Biochemistry. University of Washington, Seattle WA.
Andrea E WillsDepartment of Biochemistry. University of Washington, Seattle WA.ORCID 0000-0003-3647-8105

Funding

Transcriptional regulatory mechanisms of vertebrate regenerationR01NS099124 · NINDS · UNIVERSITY OF WASHINGTON · PI Andrea Elizabeth Wills · 2017 to 2026
$3.3M
Structure and function of metabolic enzyme assembliesR35GM149542 · NIGMS · UNIVERSITY OF WASHINGTON · PI Justin M Kollman · 2023 to 2026
$2.2M
Decoding the metabolic requirements for vertebrate appendage regenerationR01GM148490 · NIGMS · UNIVERSITY OF WASHINGTON · PI Andrea Elizabeth Wills · 2023 to 2026
$1.7M
NIGMS NIH HHS R01 GM148490NIGMS NIH HHS R35 GM149542NINDS NIH HHS R01 NS099124
6 · The paper itself

Abstract

Regeneration of lost tissue requires biosynthesis of metabolites needed for cell proliferation and growth. Among these are the critical purine nucleotides ATP and GTP. The abundance and balance of these purines is regulated by inosine monophosphate dehydrogenase 2 (IMPDH2), which catalyzes the committing step of GTP synthesis. IMPDH2 assembles into filaments that resist allosteric inhibition under conditions of high GTP demand. Here we asked whether IMPDH2 is required in the highly proliferative context of regeneration, and whether its assembly into filaments takes place in regenerating tissue. We find that inhibition of IMPDH2 leads to impaired tail regeneration and reduced cell proliferation in the tadpole

Indexed as

enzyme filamentIMPDH2nucleotide metabolismpurineregenerationXenopus

Identifiers

PMID39131357
PMCPMC11312571

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.