Evidence map›Paper›PMID 39131343›Full record

ArticlebioRxiv : the preprint server for biology2024

CIB2 function is distinct from Whirlin in the development of cochlear stereocilia staircase pattern.

Arnaud P J Giese, Andrew Parker, Sakina Rehman, Steve D M Brown, Saima Riazuddin, Craig W Vander Kooi, Michael R Bowl, Zubair M Ahmed

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Arnaud P J GieseDepartment of Otorhinolaryngology - Head & Neck Surgery, University of Maryland School of Medicine, Baltimore, MD, USA.
Andrew ParkerMRC Harwell Institute, Mammalian Genetics Unit, Harwell Campus, Oxfordshire, OX11 0RD, UK.
Sakina RehmanDepartment of Otorhinolaryngology - Head & Neck Surgery, University of Maryland School of Medicine, Baltimore, MD, USA.
Steve D M BrownMRC Harwell Institute, Mammalian Genetics Unit, Harwell Campus, Oxfordshire, OX11 0RD, UK.
Saima RiazuddinDepartment of Otorhinolaryngology - Head & Neck Surgery, University of Maryland School of Medicine, Baltimore, MD, USA.
Craig W Vander KooiDepartment of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL, USA.
Michael R BowlMRC Harwell Institute, Mammalian Genetics Unit, Harwell Campus, Oxfordshire, OX11 0RD, UK.
Zubair M AhmedDepartment of Otorhinolaryngology - Head & Neck Surgery, University of Maryland School of Medicine, Baltimore, MD, USA.ORCID 0000-0003-2914-4502

Funding

Usher Proteins in the Inner Ear Structure and FunctionR01DC012564 · NIDCD · UNIVERSITY OF MARYLAND BALTIMORE · PI Zubair M. Ahmed, Gregory I Frolenkov · 2013 to 2026
$7.0M
GIPC3, multifunctional myosin adaptor in mammalian auditory hair cellsR01DC019054 · NIDCD · UNIVERSITY OF KENTUCKY · PI FROLENKOV, GREGORY I, VANDER KOOI, CRAIG · 2020 to 2024
$2.8M
NIDCD NIH HHS R01 DC012564NIDCD NIH HHS R01 DC019054
6 · The paper itself

Abstract

Variations in genes coding for calcium and integrin binding protein 2 (CIB2) and whirlin cause deafness both in humans and mice. We previously reported that CIB2 binds to whirlin, and is essential for normal staircase architecture of auditory hair cells stereocilia. Here, we refine the interacting domains between these proteins and provide evidence that both proteins have distinct role in the development and organization of stereocilia bundles required for auditory transduction. Using a series of CIB2 and whirlin deletion constructs and nanoscale pulldown (NanoSPD) assays, we localized the regions of CIB2 that are critical for interaction with whirlin. AlphaFold 2 multimer, independently identified the same interacting regions between CIB2 and whirlin proteins, providing a detailed structural model of the interaction between the CIB2 EF2 domain and whirlin HHD2 domain. Next, we investigated genetic interaction between murine

Indexed as

CIB2cochleainner ear hair cellsstereocilia staircaseWhirlin

Identifiers

PMID39131343
PMCPMC11312573

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.