Evidence map›Paper›PMID 39128999›Full record

ArticleBMC veterinary research2024

Seminal plasma removal for medium-term preservation of ram sperm at 5 °C.

Marta Neila-Montero, Mercedes Alvarez, Marta F Riesco, Cristina Soriano-Úbeda, Rafael Montes-Garrido, Cristina Palacin-Martinez, Paulino de Paz, Luis Anel, Luis Anel-Lopez

Abstract read
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In one paragraph

Article in BMC veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Marta Neila-MonteroItra-ULE, INDEGSAL, University of León, Campus de Vegazana S/N, León, 24071, Spain.
Mercedes AlvarezItra-ULE, INDEGSAL, University of León, Campus de Vegazana S/N, León, 24071, Spain. mmalvg@unileon.es.
Marta F RiescoItra-ULE, INDEGSAL, University of León, Campus de Vegazana S/N, León, 24071, Spain.
Cristina Soriano-ÚbedaItra-ULE, INDEGSAL, University of León, Campus de Vegazana S/N, León, 24071, Spain.
Rafael Montes-GarridoItra-ULE, INDEGSAL, University of León, Campus de Vegazana S/N, León, 24071, Spain.
Cristina Palacin-MartinezItra-ULE, INDEGSAL, University of León, Campus de Vegazana S/N, León, 24071, Spain.
Paulino de PazItra-ULE, INDEGSAL, University of León, Campus de Vegazana S/N, León, 24071, Spain.
Luis AnelItra-ULE, INDEGSAL, University of León, Campus de Vegazana S/N, León, 24071, Spain.
Luis Anel-LopezItra-ULE, INDEGSAL, University of León, Campus de Vegazana S/N, León, 24071, Spain.

Funding

Junta de Castilla y León ORDEN EDU/556/2019Ministerio de Economía y Competitividad PRE2018-086400Ministerio de Educación y Formación Profesional FPU17/04142
6 · The paper itself

Abstract

This study aimed to investigate if washing ram sperm from seminal plasma (SP) could be an effective tool to extend sperm lifespan in medium-term preservation in liquid form to optimize ovine artificial insemination protocols. To this end, in Experiment 1 SP was added to a sperm model without previous contact with this substance (ram epididymal sperm) at the beginning or the end of a 48-hour preservation protocol at 5 °C (n = 13). Sperm motility and kinetic parameters and sperm functionality in terms of sperm viability, apoptosis, mitochondrial activity and reacted acrosomes were assessed after 6 h of storage at 15 °C (standard liquid preservation method) and 24 and 48 h at 5 °C. Extended sperm showed better results after 48 h when stored in the absence than in the presence of SP in most sperm quality parameters. Moreover, the final SP supplementation of this experimental group resulted in the highest sperm motility and kinetic parameters, viability and mitochondrial activity. These results suggested that initial SP deprivation could be beneficial in a medium-term ram sperm preservation protocol in liquid form, as well as a final supplementation. Therefore, we conducted Experiment 2 to evaluate the effect of SP removal from freshly ejaculated ram semen under the same storage conditions as in Experiment 1 (n = 12). Surprisingly, SP withdrawal impaired sperm functionality, leading to increased apoptosis and decreased mitochondrial activity after 24 and 48 h at 5 °C. Conversely, SP supplementation at the end of the preservation protocol of the ejaculate processed as usual had a positive effect on sperm quality and fertility. To summarize, SP absence was beneficial for a medium-term preservation protocol (up to 48 h at 5 °C) of ram epididymal sperm, but the same preservation protocol for ram ejaculated sperm revealed a possible failure of the SP removal method in avoiding the sperm-SP interaction effect. Meanwhile, SP supplementation of ram semen at the end of the preservation protocol increased in vitro sperm quality and fertility after artificial insemination.

Indexed as

SemenSemen PreservationSpermatozoaSperm MotilityAnimalsInsemination, ArtificialMaleSemen AnalysisSheepChilled semenCooled semenEpididymal spermOvineSemen preservation

Identifiers

PMID39128999
PMCPMC11318144

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.