ArticleNature communications2024
The gradual establishment of complex coumarin biosynthetic pathway in Apiaceae.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
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- Elucidating the Furanocoumarin Biosynthetic Pathway inPlants (Basel, Switzerland) · 2026Article
- The role of coumarin biosynthesis in modulating plant drought responses: insights from PpBMT overexpression in Arabidopsis.BMC plant biology · 2026Article
- Evolutionary tuning of a biosynthetic gene cluster drives furanocoumarin accumulation and diversification.Nature communications · 2026Article
- Coumarin metabolites in Ocimum: chemical diversity, biosynthetic pathways, and network pharmacology-based prediction of multi-target anticancer potential.Plant molecular biology · 2026Article
- Coumarin derivatives as HIV-1 inhibitors: mechanistic insights and structure-activity relationships.Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents · 2026Review
- The targeted metabolomic profile of laticifers in rubber tree.Journal of integrative plant biology · 2026Article
- Integrated Metabolome and Transcriptome Analysis in Ammi majus L. Revealed Candidate Genes of Furanocoumarins Pathway.Biochemical genetics · 2026Article
- Rhizobacteria opportunistically boost colonization and impair plant fitness by degrading plant-derived coumarins under iron deficiency.Nature communications · 2026Article
- Growth Year and Chemotype Synergistically Regulate Coumarin Accumulation and the Associated Transcriptional Profiles inPlants (Basel, Switzerland) · 2026Article
- Discover the maze-like network for glabridin biosynthesis.Nature communications · 2026Article
- Quality formation inFrontiers in plant science · 2026Review
- Herbgenomics: Unraveling natural product biosynthesis in traditional Chinese medicine.Chinese herbal medicines · 2026Article
- Harnessing coumarin-thio(seleno)cyanate conjugates: potentJournal of enzyme inhibition and medicinal chemistry · 2025Article
- Evolution of terpene synthases in the sesquiterpene biosynthesis pathway and analysis of their transcriptional regulatory network in Asteraceae.Horticulture research · 2025Article
- Metabolomic Profiling of Citrus Grafts Challenged byJournal of agricultural and food chemistry · 2025Article
- Microminutinin, a Fused Bis-Furan Coumarin fromPlants (Basel, Switzerland) · 2025Article
- Spatiotemporal Distribution Patterns of Osthole and Expression Correlation of theInternational journal of molecular sciences · 2025Article
- Are Furanocoumarins Present in the Cichorieae Tribe of Asteraceae? A Comparative Study ofPlants (Basel, Switzerland) · 2025Article
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10 authors.
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Abstract
Complex coumarins (CCs) represent characteristic metabolites found in Apiaceae plants, possessing significant medical value. Their essential functional role is likely as protectants against pathogens and regulators responding to environmental stimuli. Utilizing genomes and transcriptomes from 34 Apiaceae plants, including our recently sequenced Peucedanum praeruptorum, we conduct comprehensive phylogenetic analyses to reconstruct the detailed evolutionary process of the CC biosynthetic pathway in Apiaceae. Our results show that three key enzymes - p-coumaroyl CoA 2'-hydroxylase (C2'H), C-prenyltransferase (C-PT), and cyclase - originated successively at different evolutionary nodes within Apiaceae through various means of gene duplications: ectopic and tandem duplications. Neofunctionalization endows these enzymes with novel functions necessary for CC biosynthesis, thus completing the pathway. Candidate genes are cloned for heterologous expression and subjected to in vitro enzymatic assays to test our hypothesis regarding the origins of the key enzymes, and the results precisely validate our evolutionary inferences. Among the three enzymes, C-PTs are likely the primary determinant of the structural diversity of CCs (linear/angular), due to divergent activities evolved to target different positions (C-6 or C-8) of umbelliferone. A key amino acid variation (Ala161/Thr161) is identified and proven to play a crucial role in the alteration of enzymatic activity, possibly resulting in distinct binding forms between enzymes and substrates, thereby leading to different products. In conclusion, this study provides a detailed trajectory for the establishment and evolution of the CC biosynthetic pathway in Apiaceae. It explains why only a portion, not all, of Apiaceae plants can produce CCs and reveals the mechanisms of CC structural diversity among different Apiaceae plants.
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