Evidence map›Paper›PMID 39127235›Full record

ArticleExperimental eye research2024

Comparative proteomic study of retinal ganglion cells undergoing various types of cellular stressors.

Christopher R Starr, James A Mobley, Marina S Gorbatyuk

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Experimental eye research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors.

Christopher R StarrDepartment of Optometry and Vision Science, University of Alabama at Birmingham, Birmingham, AL, USA, 35233.
James A MobleyDepartment of Optometry and Vision Science, University of Alabama at Birmingham, Birmingham, AL, USA, 35233.
Marina S GorbatyukDepartment of Optometry and Vision Science, University of Alabama at Birmingham, Birmingham, AL, USA, 35233. Electronic address: mgortk@uab.edu.

Funding

Post-translational histone modification in ocular tissues of mice exposed to arsenicalsR01EY027763 · NEI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI GORBATYUK, MARINA · 2018 to 2022
$2.0M
RXR-based therapy for diabetic retinopathyR01EY035539 · NEI · WAKE FOREST UNIVERSITY HEALTH SCIENCES · PI Marina Gorbatyuk, Maria Bartolomeo Grant · 2024 to 2026
$1.7M
NEI NIH HHS R01 EY027763NEI NIH HHS R01 EY035539
6 · The paper itself

Abstract

Retinal ganglion cell (RGC) damage serves as a key indicator of various retinal degenerative diseases, including diabetic retinopathy (DR), glaucoma, retinal arterial and retinal vein occlusions, as well as inflammatory and traumatic optic neuropathies. Despite the growing body of data on the RGC proteomics associated with these conditions, there has been no dedicated study conducted to compare the molecular signaling pathways involved in the mechanism of neuronal cell death. Therefore, we launched the study using two different insults leading to RGC death: glutamate excitotoxicity and optic nerve crush (ONC). C57BL/6 mice were used for the study and underwent NMDA- and ONC-induced damage. Twenty-four hours after ONC and 1 h after NMDA injection, we collected RGCs using CD90.2 coupled magnetic beads, prepared protein extracts, and employed LC-MS for the global proteomic analysis of RGCs. Statistically significant changes in proteins were analyzed to identify changes to cellular signaling resulting from the treatment. We identified unique and common alterations in protein profiles in RGCs undergoing different types of cellular stresses. Our study not only identified both unique and shared proteomic changes but also laid the groundwork for the future development of a therapeutic platform for testing gene candidates for DR and glaucoma.

Indexed as

Mice, Inbred C57BLOptic Nerve InjuriesProteomicsRetinal Ganglion CellsAnimalsChromatography, LiquidDisease Models, AnimalEye ProteinsMiceNerve CrushN-MethylaspartateProteomeEye ProteinsN-MethylaspartateProteomeExcitotoxicityGlaucomaOphthalmologyOptic neuropathyProteomicsRetina

Identifiers

PMID39127235

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.