Evidence map›Paper›PMID 39126474›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Visualization and Quantification of Rapid Chromosome Movements at Early Stages of Mouse Meiosis.

Luciana Previato de Almeida, Chih-Ying Lee, Agustin Carbajal, Rodrigo O de Castro, Roberto J Pezza

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Luciana Previato de AlmeidaCell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
Chih-Ying LeeCell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
Agustin CarbajalCell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
Rodrigo O de CastroCell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
Roberto J PezzaCell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA. pezzar@omrf.org.

Funding

Epigenetic control of meiotic recombination in mammals - Equipment SupplementR01GM125803 · NIGMS · OKLAHOMA MEDICAL RESEARCH FOUNDATION · PI PEZZA, ROBERTO JOSE · 2018 to 2021
$1.8M
Development of a lacO/lacI based flourescence reporter-operator system to study chromosome dynamics in miceR21HD103562 · NICHD · OKLAHOMA MEDICAL RESEARCH FOUNDATION · PI PEZZA, ROBERTO JOSE · 2021 to 2022
$495k
NICHD NIH HHS R21 HD103562NIGMS NIH HHS R01 GM125803
6 · The paper itself

Abstract

Telomere-led rapid chromosome movements (RPMs) are a conserved characteristic of chromosome dynamics in meiosis. RPMs have been suggested to influence critical meiotic functions such as DNA repair and the association of the homologous chromosomes. Here, we describe a method using 3D time-lapse fluorescence imaging to monitor RPMs in Hoechst-stained mouse seminiferous tubules explants. We supplement visualization with customized quantitative motion analysis and in silico simulation. The ability to carry out live imaging, combined with quantitative image analysis, offers a sensitive tool to investigate the regulation of RPMs, chromosome reorganizations that precede dynamic mid-prophase events, and their contribution to faithful transmission of genetic information.

Indexed as

MeiosisAnimalsChromosomesMaleMiceSeminiferous TubulesTelomereTime-Lapse ImagingChromosome dynamicsMicroscopyMouse gametogenesisSpermatocyteTelomere-led rapid prophase movements

Identifiers

PMID39126474
PMCPMC12207911

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.