Evidence map›Paper›PMID 39126464›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

CC-seq: Nucleotide-Resolution Mapping of Spo11 DNA Double-Strand Breaks in S. cerevisiae Cells.

George G B Brown, Will H Gittens, Rachal M Allison, Antony W Oliver, Matthew J Neale

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

George G B BrownGenome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, UK. gb305@sussex.ac.uk.
Will H GittensGenome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, UK.
Rachal M AllisonGenome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, UK.
Antony W OliverGenome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, UK.
Matthew J NealeGenome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, UK. m.neale@sussex.ac.uk.

Funding

Wellcome Trust 200843/Z/16/ZWellcome Trust 225852/Z/22/Z
6 · The paper itself

Abstract

During meiosis, Spo11 generates DNA double-strand breaks to induce recombination, becoming covalently attached to the 5' ends on both sides of the break during this process. Such Spo11 "covalent complexes" are transient in wild-type cells, but accumulate in nuclease mutants unable to initiate repair. The CC-seq method presented here details how to map the location of these Spo11 complexes genome-wide with strand-specific nucleotide-resolution accuracy in synchronized Saccharomyces cerevisiae meiotic cells.

Indexed as

DNA Breaks, Double-StrandedEndodeoxyribonucleasesMeiosisSaccharomyces cerevisiaeDNA, FungalDNA RepairMeiotic Recombination Protein SPO11Saccharomyces cerevisiae ProteinsSequence Analysis, DNADNA, FungalEndodeoxyribonucleasesMeiotic Recombination Protein SPO11Saccharomyces cerevisiae ProteinsSpo11 protein, S cerevisiaeCCCC-seqChromosomeCovalent complexDNA breakDSBGenome-wideMappingMeiosisNext-generation sequencingNGSRecombinationSequencingSingle nucleotideSpo11

Identifiers

PMID39126464

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.