Evidence map›Paper›PMID 39125852›Full record

ArticleInternational journal of molecular sciences2024

A DAMP-Based Assay for Rapid and Affordable Diagnosis of Bacterial Meningitis Agents:

Liubov A Shkodenko, Al-Abbass Mohamed, Muhannad Ateiah, Maria S Rubel, Elena I Koshel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Liubov A ShkodenkoLaboratory of DNA-Nanosensor Diagnostics, ITMO University, Lomonosova Street, 9, 191002 St Petersburg, Russia.ORCID 0000-0002-4978-8535
Al-Abbass MohamedLaboratory of DNA-Nanosensor Diagnostics, ITMO University, Lomonosova Street, 9, 191002 St Petersburg, Russia.ORCID 0000-0002-7167-8690
Muhannad AteiahLaboratory of DNA-Nanosensor Diagnostics, ITMO University, Lomonosova Street, 9, 191002 St Petersburg, Russia.ORCID 0000-0002-2308-9605
Maria S RubelLaboratory of DNA-Nanosensor Diagnostics, ITMO University, Lomonosova Street, 9, 191002 St Petersburg, Russia.ORCID 0000-0002-5991-6772
Elena I KoshelLaboratory of DNA-Nanosensor Diagnostics, ITMO University, Lomonosova Street, 9, 191002 St Petersburg, Russia.ORCID 0000-0002-8957-5956

Funding

Russian Science Foundation 22-75-10073
6 · The paper itself

Abstract

The rapid and accurate diagnosis of meningitis is critical for preventing severe complications and fatalities. This study addresses the need for accessible diagnostics in the absence of specialized equipment by developing a novel diagnostic assay. The assay utilizes dual-priming isothermal amplification (DAMP) with unique internal primers to significantly reduce non-specificity. For fluorescence detection, the dye was selected among Brilliant Green, Thioflavin T, and dsGreen. Brilliant Green is preferred for this assay due to its availability, high fluorescence level, and optimal sample-to-background (S/B) ratio. The assay was developed for the detection of the primary causative agents of meningitis (

Indexed as

Haemophilus influenzaeMeningitis, BacterialMolecular Diagnostic TechniquesNeisseria meningitidisNucleic Acid Amplification TechniquesStreptococcus pneumoniaeHumansSensitivity and SpecificityCNS infectionsDAMPdiagnostic assayDNA diagnosticsisotheral amplificationmeningitisPOC

Identifiers

PMID39125852
PMCPMC11311791

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.