Evidence map›Paper›PMID 39122354›Full record

ArticleThe British journal of ophthalmology2024

Deep sequencing as a diagnostic tool in patients with suspected primary vitreoretinal lymphoma.

Charlene Choo, Olivia Cote, Karina Bostwick, Matthew Regueiro, Jill Wells, Hans E Grossniklaus, John Gonzales, Steven Yeh, Armin Hinterwirth, Thuy Doan and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The British journal of ophthalmology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Charlene ChooFrancis I Proctor Foundation, San Francisco, California, USA.ORCID http://orcid.org/0000-0001-8626-4004
Olivia CoteEmory University School of Medicine, Atlanta, Georgia, USA.
Karina BostwickEmory Eye Center, Atlanta, Georgia, USA.
Matthew RegueiroCleveland Clinic Cole Eye Institute, Cleveland, Ohio, USA.
Jill WellsEmory Eye Center, Atlanta, Georgia, USA.
Hans E GrossniklausEmory Eye Center, Atlanta, Georgia, USA.
John GonzalesFrancis I Proctor Foundation, San Francisco, California, USA.ORCID http://orcid.org/0000-0003-0558-9793
Steven YehDepartment of Ophthalmology and Visual Sciences, Truhlsen Eye Institute, University of Nebraska Medical Center, Omaha, Nebraska, USA.
Armin HinterwirthFrancis I Proctor Foundation, San Francisco, California, USA.
Thuy DoanFrancis I Proctor Foundation, San Francisco, California, USA.
Jessica G ShanthaFrancis I Proctor Foundation, San Francisco, California, USA jessica.shantha@ucsf.edu.

Funding

Rapid-Prototyping and Design CoreP30EY002162 · NEI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ULLIAN, ERIK M · 1985 to 2024
$16.8M
P30-Core Grant for Vision Research Core CP30EY006360 · NEI · EMORY UNIVERSITY · PI BOATRIGHT, JEFFREY H · 1986 to 2025
$15.3M
Ophthalmic Disease and Laboratory Surveillance in a Viral Hemorrhagic Fever ZoneK23EY030158 · NEI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI SHANTHA, JESSICA · 2019 to 2023
$1.2M
NEI NIH HHS K23 EY030158NEI NIH HHS P30 EY002162NEI NIH HHS P30 EY006360
6 · The paper itself

Abstract

purposeTo compare the diagnostic utility of metagenomic deep sequencing (MDS) to cytology, flow cytometry and gene rearrangement by PCR in ocular samples of patients with suspected vitreoretinal lymphoma (VRL).

methodsPatients with suspected VRL underwent ocular sampling of one or both eyes at the Emory Eye Center from September 2017 to June 2022. Ocular samples were evaluated with MDS and conventional diagnostics. MDS was performed at the Ralph and Sophie Heintz Laboratory at the F.I. Proctor Foundation. Relevant demographic and clinical data were retrospectively collected from medical records. Patients were diagnosed with VRL based on clinical assessment and conventional diagnostic testing.

resultsThis study included 13 patients with suspected VRL who underwent diagnostic vitrectomy, including 1 patient who had an additional subretinal biopsy. Six patients (46.2%) were diagnosed with VRL. Among patients diagnosed with VRL, MDS detected pathogenic mutations in 5 out of 6 patients (83.3%) while cytology was positive for VRL in 4 out of 6 patients (66.7%), flow cytometry in 4 out of 4 patients (100.0%) and PCR in 4 out of 4 patients (100.0%). MDS detected mutations in DISCUSSION: MDS detected pathogenic mutations in five out of six patients diagnosed with VRL, including in two patients with negative cytology, demonstrating its potential to improve diagnostic rates of VRL as an adjunctive test.

Indexed as

Flow CytometryHigh-Throughput Nucleotide SequencingIntraocular LymphomaRetinal NeoplasmsVitreous BodyAdultAgedAged, 80 and overFemaleHumansMaleMetagenomicsMiddle AgedPolymerase Chain ReactionRetrospective StudiesVitrectomyDiagnostic tests/InvestigationGeneticsInflammationNeoplasiaOphthalmologic Surgical Procedures

Identifiers

PMID39122354
PMCPMC11652249

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.