Evidence map›Paper›PMID 39120764›Full record

ArticleClinical oral investigations2024

Anti-inflammatory potential of casein enzymatic hydrolysate/gelatin methacryloyl scaffolds for vital pulp therapy.

Payam Paymanpour, Caroline Anselmi, Lais M Cardoso, Ana Beatriz Gomes de Carvalho, Igor Paulino Mendes Soares, Josimeri Hebling, Renan Dal-Fabbro, Marco C Bottino

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical oral investigations, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Bilayer gelatin-methacryloyl scaffold for pulp inflammation suppression and dentin-like tissue regeneration.Journal of controlled release : official journal of the Controlled Release Society · 2026
    Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Payam PaymanpourDepartment of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, 1011 N. University, Ann Arbor, MI, 48109, USA.
Caroline AnselmiDepartment of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, 1011 N. University, Ann Arbor, MI, 48109, USA.
Lais M CardosoDepartment of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, 1011 N. University, Ann Arbor, MI, 48109, USA.
Ana Beatriz Gomes de CarvalhoDepartment of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, 1011 N. University, Ann Arbor, MI, 48109, USA.
Igor Paulino Mendes SoaresDepartment of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, 1011 N. University, Ann Arbor, MI, 48109, USA.
Josimeri HeblingDepartment of Morphology and Pediatric Dentistry, São Paulo State University, Araraquara, SP, Brazil.
Renan Dal-FabbroDepartment of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, 1011 N. University, Ann Arbor, MI, 48109, USA.
Marco C BottinoDepartment of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, 1011 N. University, Ann Arbor, MI, 48109, USA. mbottino@umich.edu.

Funding

Personalized Strategies for Periodontal Tissue Regeneration - A Converged Biofabrication ApproachR01DE031476 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Marco C Bottino · 2022 to 2026
$2.6M
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 88887.685220/2022-00NIDCR NIH HHS R01 DE031476NIDCR NIH HHS R01DE031476
6 · The paper itself

Abstract

objectivesTo synthesize casein enzymatic hydrolysate (CEH)-laden gelatin methacryloyl (GelMA) fibrous scaffolds and evaluate the cytocompatibility and anti-inflammatory effects on dental pulp stem cells (DPSCs). MATERIALS AND

methodsGelMA fibrous scaffolds with 10%, 20%, and 30% CEH (w/w) and without CEH (control) were obtained via electrospinning. Chemo-morphological, degradation, and mechanical analyses were conducted to evaluate the morphology and composition of the fibers, mass loss, and mechanical properties, respectively. Adhesion/spreading and viability of DPSCs seeded on the scaffolds were also assessed. The anti-inflammatory potential on DPSCs was tested after the chronic challenge of cells with lipopolysaccharides (LPS), followed by treatment with extracts obtained after immersing the scaffolds in α-MEM. The synthesis of the pro-inflammatory cytokines IL-6, IL-1α, and TNF-α was measured by ELISA. Data were analyzed by ANOVA/post-hoc tests (α = 5%).

resultsCEH-laden electrospun fibers had a larger diameter than pure GelMA (p ≤ 0.036). GelMA scaffolds laden with 20% and 30% CEH had a greater mass loss. Tensile strength was reduced for the 10% CEH fibers (p = 0.0052), whereas no difference was observed for the 20% and 30% fibers (p ≥ 0.6736) compared to the control. Young's modulus decreased with CEH (p < 0.0001). Elongation at break increased for the 20% and 30% CEH scaffolds (p ≤ 0.0038). Over time, DPSCs viability increased across all groups, indicating cytocompatibility, with CEH-laden scaffolds exhibiting greater cell viability after seven days (p ≤ 0.0166). Also, 10% CEH-GelMA scaffolds decreased the IL-6, IL-1α, and TNF-α synthesis (p ≤ 0.035).

conclusionCEH-laden GelMA scaffolds facilitated both adhesion and proliferation of DPSCs, and 10% CEH provided anti-inflammatory potential after chronic LPS challenge. CLINICAL RELEVANCE: CEH incorporated in GelMA fibrous scaffolds demonstrated the potential to be used as a cytocompatible and anti-inflammatory biomaterial for vital pulp therapy.

Indexed as

Anti-Inflammatory AgentsCaseinsCell SurvivalDental PulpGelatinTissue ScaffoldsBiocompatible MaterialsCell AdhesionCells, CulturedCytokinesEnzyme-Linked Immunosorbent AssayHumansMaterials TestingMethacrylatesStem CellsSurface PropertiesAnti-Inflammatory AgentsBiocompatible MaterialsCaseinsCytokinesGelatingelatin methacryloylMethacrylatesAnti-inflammatory agentsCaseinsDental Pulp CappingDrug deliveryRegenerative endodontics

Identifiers

PMID39120764
PMCPMC13022930

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.