Evidence map›Paper›PMID 39119722›Full record

ArticleEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin2024

Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024.

Leonard Schuele, Leandre Murhula Masirika, Jean Claude Udahemuka, Freddy Belesi Siangoli, Justin Bengehya Mbiribindi, Pacifique Ndishimye, Frank M Aarestrup, Marion Koopmans, Bas B Oude Munnink, Richard Molenkamp and 2 more

Erratum issuedAbstract read
In one paragraph

Article in Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. A rapidly developing outbreak of clade Ib mpox among gay, bisexual and other men who have sex with men associated with severe proctitis, Northern Ireland, June 2026.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2026
    Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. An atypical case of infection with the Mpox virus clade IIb lineage C.1 - clinical and virological findings.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026
    Article
  11. Review
  12. Article
  13. Multiplex PCR to Differentiate Monkeypox Virus Clades.Emerging infectious diseases · 2026
    Article
  14. Article
  15. Article
  16. Nosocomial transmission in a monkeypox virus clade Ib outbreak, Ireland, August to October 2025.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025
    Article
  17. Review
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Leonard SchueleDepartment of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands.
Leandre Murhula MasirikaThese authors contributed equally to this work and share first authorship.
Jean Claude UdahemukaThese authors contributed equally to this work and share first authorship.
Freddy Belesi SiangoliDivision Provinciale de la Santé, South Kivu, Bukavu, Democratic Republic of the Congo.
Justin Bengehya MbiribindiDivision Provinciale de la Santé, South Kivu, Bukavu, Democratic Republic of the Congo.
Pacifique NdishimyeGenomics Research and Development Division, Stansile Research Organization, Kigali, Rwanda.
Frank M AarestrupResearch Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark.
Marion KoopmansDepartment of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands.
Bas B Oude MunninkDepartment of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands.
Richard MolenkampDepartment of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands.
GREATLIFE MPOX groupThe members of the network are listed under Collaborators.
Collaborators

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.

Indexed as

Monkeypox virusMpox, MonkeypoxReal-Time Polymerase Chain ReactionSensitivity and SpecificityDNA, ViralHumansPhylogenyDNA, Viraldiagnostic testMonkeypox virusMonkeypox virus Clade IbMpox virus Clade IbReal-time PCR

Identifiers

PMID39119722
PMCPMC11312019

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.