Evidence map›Paper›PMID 39117030›Full record

ArticleDevelopmental biology2024

Visualizing metabolic regulation using metabolic biosensors during sea urchin embryogenesis.

Aidan Furze, Ashley Waldron, Mamiko Yajima

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Aidan FurzeDepartment of Molecular Biology Cell Biology Biochemistry, Brown University, 185 Meeting Street, BOX-GL277, Providence, RI, 02912, USA.
Ashley WaldronDepartment of Molecular Biology Cell Biology Biochemistry, Brown University, 185 Meeting Street, BOX-GL277, Providence, RI, 02912, USA.
Mamiko YajimaDepartment of Molecular Biology Cell Biology Biochemistry, Brown University, 185 Meeting Street, BOX-GL277, Providence, RI, 02912, USA. Electronic address: Mamiko_Yajima@brown.edu.

Funding

Functional contribution of Metabolism in embryonic developmentR21HD109132 · NICHD · BROWN UNIVERSITY · PI YAJIMA, MAMIKO · 2022 to 2023
$439k
NICHD NIH HHS R21 HD109132
6 · The paper itself

Abstract

Growing evidence suggests that metabolic regulation directly influences cellular function and development and thus may be more dynamic than previously expected. In vivo and in real-time analysis of metabolite activities during development is crucial to test this idea directly. In this study, we employ two metabolic biosensors to track the dynamics of pyruvate and oxidative phosphorylation (Oxphos) during the early embryogenesis of the sea urchin. A pyruvate sensor, PyronicSF, shows the signal enrichment on the mitotic apparatus, which is consistent with the localization patterns of the corresponding enzyme, pyruvate kinase (PKM). The addition of pyruvate increases the PyronicSF signal, while PKM knockdown decreases its signal, responding to the pyruvate level in the cell. Similarly, a ratio-metric sensor, Grx-roGFP, that reads the redox potential of the cell responds to DTT and H

Indexed as

Biosensing TechniquesEmbryonic DevelopmentOxidative PhosphorylationPyruvate KinasePyruvic AcidSea UrchinsAnimalsEmbryo, NonmammalianTime-Lapse ImagingPyruvate KinasePyruvic AcidEmbryonic developmentMetabolic regulationPyruvate sensorSea urchin

Identifiers

PMID39117030
PMCPMC11402557

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.