Evidence map›Paper›PMID 39116876›Full record

ArticleDevelopmental cell2024

Hox gene activity directs physical forces to differentially shape chick small and large intestinal epithelia.

Hasreet K Gill, Sifan Yin, Nandan L Nerurkar, John C Lawlor, ChangHee Lee, Tyler R Huycke, L Mahadevan, Clifford J Tabin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Uterine organoids reveal insights into epithelial specification and plasticity in development and disease.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hasreet K GillDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA.
Sifan YinHarvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
Nandan L NerurkarThe Fu Foundation School of Engineering and Applied Science, Columbia University, New York, NY 10027, USA.
John C LawlorDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA.
ChangHee LeeDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA.
Tyler R HuyckeDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA.
L MahadevanHarvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA; Department of Physics, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA. Electronic address: lm@seas.harvard.edu.
Clifford J TabinDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA. Electronic address: tabin@genetics.med.harvard.edu.

Funding

The Organoid and Cell Culture CoreP30DK132710 · NIDDK · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Robert F. Schwabe · 2022 to 2026
$7.2M
Signals and mechanical forces controlling radial gut morphogenesisR01HD087234 · NICHD · HARVARD MEDICAL SCHOOL · PI TABIN, CLIFFORD J. · 2016 to 2025
$3.2M
Integration of Mechanical Forces and Signaling in the Morphogenesis of the GutR01HD089934 · NICHD · HARVARD MEDICAL SCHOOL · PI TABIN, CLIFFORD J. · 2017 to 2021
$2.0M
NICHD NIH HHS R01 HD087234NICHD NIH HHS R01 HD089934NIDDK NIH HHS P30 DK132710
6 · The paper itself

Abstract

Hox transcription factors play crucial roles in organizing developmental patterning across metazoa, but how these factors trigger regional morphogenesis has largely remained a mystery. In the developing gut, Hox genes help demarcate identities of intestinal subregions early in embryogenesis, which ultimately leads to their specialization in both form and function. Although the midgut forms villi, the hindgut develops sulci that resolve into heterogeneous outgrowths. Combining mechanical measurements of the embryonic chick intestine and mathematical modeling, we demonstrate that the posterior Hox gene HOXD13 regulates biophysical phenomena that shape the hindgut lumen. We further show that HOXD13 acts through the transforming growth factor β (TGF-β) pathway to thicken, stiffen, and promote isotropic growth of the subepithelial mesenchyme-together, these features lead to hindgut-specific surface buckling. TGF-β, in turn, promotes collagen deposition to affect mesenchymal geometry and growth. We thus identify a cascade of events downstream of positional identity that direct posterior intestinal morphogenesis.

Indexed as

Gene Expression Regulation, DevelopmentalHomeodomain ProteinsIntestinal MucosaMesodermMorphogenesisTransforming Growth Factor betaAnimalsBody PatterningChick EmbryoChickensGenes, HomeoboxIntestine, LargeIntestine, SmallSignal TransductionHomeodomain ProteinsTransforming Growth Factor betagutHox genesmechanical forcesmodelingmorphogenesisTGF-β pathway

Identifiers

PMID39116876
PMCPMC11537829

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.