Evidence map›Paper›PMID 39115808›Full record

ArticleImmunological investigations2024

A Comprehensive Protocol for the Collection, Differentiation, Cryopreservation, and Resuscitation of Primary Murine Bone Marrow Derived Macrophages (BMDM).

Abby M Luu, Kelly M Shepardson, Agnieszka Rynda-Apple

Abstract read
In one paragraph

Article in Immunological investigations, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Abby M LuuDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, Montana, USA.
Kelly M ShepardsonDepartment of Molecular Cell Biology, University of California Merced, Merced, California, USA.
Agnieszka Rynda-AppleDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, Montana, USA.

Funding

Translational Biomarkers CoreP20GM104417 · NIGMS · MONTANA STATE UNIVERSITY - BOZEMAN · PI ADAMS, ALEXANDRA K. · 2014 to 2023
$23.1M
NRSA Training CoreTL1TR002318 · NCATS · UNIVERSITY OF WASHINGTON · PI Megan Moore · 2017 to 2026
$8.4M
Pilot Grants ProgramP30GM110732 · NIGMS · MONTANA STATE UNIVERSITY - BOZEMAN · PI QUINN, MARK T · 2014 to 2018
$5.4M
NCATS NIH HHS TL1 TR002318NIGMS NIH HHS P20 GM104417NIGMS NIH HHS P30 GM110732
6 · The paper itself

Abstract

backgroundThe field of immunology has undoubtedly benefited from the

methodsBone marrow cells were collected from mice with a C57BL6 genetic background. Cells were differentiated using L929 conditioned media. Cells were assessed using a combination of microscopy, differential staining, immunocytochemistry, and trypan blue. Results: Primary murine BMDMs that underwent cryopreservation followed by resuscitation retained a high degree of viability. Furthermore, these BMDMs retained on overall ability to clear

resultsPrimary murine BMDMs that underwent cryopreservation followed by resuscitation retained a high degree of viability. Furthermore, these BMDMs retained on overall ability to clear

conclusionCrypopreserved and resuscitated primary murine BMDMs were viable and retained their pverall

Indexed as

Cell DifferentiationCell SurvivalCryopreservationMacrophagesMice, Inbred C57BLAnimalsBone Marrow CellsCells, CulturedCulture Media, ConditionedMiceStaphylococcus aureusCulture Media, ConditionedBacterial burdenbone marrow derived macrophage (BMDM)cell culturecryopreservationdifferentiationfresh BMDMfrozen BMDML929primary murine cellsresuscitation

Identifiers

PMID39115808
PMCPMC11451725

What OpenQuestion holds

Textmetadata
LicenceTDM
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.