Evidence map›Paper›PMID 39115780›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

High-Pressure Freezing and Low-Temperature Processing of Seeds for Electron Microscopy and Electron Tomography.

Juan Li, Liangpeng Gou, Jinbo Shen, Wenhan Cao

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Juan LiState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, China.
Liangpeng GouState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, China.
Jinbo ShenState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, China.
Wenhan CaoState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, China. caowh@zafu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High-pressure freezing/freeze substitution has been used to preserve biological samples for ultrastructure study instead of chemical fixation. For most plant samples, the water content is too high and cannot be properly preserved during cryofixation. Additionally, the cell wall is a barrier that prevents the substitution of water with the resin. In this chapter, we will discuss modified high-pressure freezing and subsequent processing protocols based on our routinely used methodology for examining Arabidopsis seeds in transmission electron microscopy and electron tomography.

Indexed as

ArabidopsisElectron Microscope TomographySeedsCold TemperatureCryopreservationFreeze SubstitutionFreezingMicroscopy, Electron, TransmissionPressureElectron tomographyFreeze substitutionHigh-pressure freezingSecretory proteinTransmission electron microscopy

Identifiers

PMID39115780

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.