Polysaccharides Analysis of Cell Wall Using Carbohydrate Gel Electrophoresis.

Zhao Wen, Jiaxi Li, Lanjun Zhang, Baocai Zhang, Yihua Zhou

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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 authors.

Zhao WenCenter for Genome Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

Jiaxi LiCenter for Genome Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

Lanjun ZhangCenter for Genome Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

Baocai ZhangCenter for Genome Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

Yihua ZhouCenter for Genome Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China. yhzhou@genetics.ac.cn.

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6 · The paper itself

Abstract

The plant cell wall is rich in polysaccharides with high heterogeneity. Investigating the composition and structure of cell wall polysaccharides is crucial for understanding the functionalities of plant cell walls. Carbohydrate electrophoresis is a sensitive and rapid method to analyze polysaccharides qualitatively and quantitatively. The process includes digesting the polysaccharides with appropriate cleavage enzymes, labeling the reducing ends of the released oligosaccharides with a highly charged fluorophore, and separating the labeled oligosaccharides in a polyacrylamide gel via high-voltage electrophoresis. The generated fluorescence can be calculated as compared to that of oligosaccharide standards. Therefore, this is a convenient method for polysaccharide characterization that can be performed in most laboratories. Here, we introduce the detailed operational steps and precautions, which are helpful for researchers to quickly obtain the structural information of polysaccharides.

Indexed as

Cell WallPolysaccharidesElectrophoresisElectrophoresis, Polyacrylamide GelOligosaccharidesOligosaccharidesPolysaccharidesANTSElectrophoresisFluorescence labelingGlycosyl hydrolaseMixed-linkage glucanPolysaccharidesXylan

Identifiers

PMID39115767

DOI10.1007/978-1-4939-6722-3_15

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