Evidence map›Paper›PMID 39115765›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Proteomic Analysis of Secreted Vesicle Proteins from the Plant Cells.

Haidi Yin, Jianying Wang, Zhong-Ping Yao

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Haidi Yin *Multi-omics Mass Spectrometry Facility, Shenzhen Medical Academy of Research and Translation, Shenzhen, Guangdong, China.
Jianying Wang *Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
Zhong-Ping YaoDepartment of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China. zhongping.yao@polyu.edu.hk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A working pipeline for proteomic analysis of secreted vesicle proteins from the plant cells has been developed using urea and mass spectrometry-compatible detergent RapiGest SF, where vesicles could be efficiently lysed and membrane-bound proteins could be efficiently dissolved and digested. The vesicle lysis and the protein digestion procedures are performed within one tube to minimize the protein loss. The protein digest is analyzed using LC-MS/MS after desalting with an SPE spin column.

Indexed as

Plant CellsPlant ProteinsProteomicsTandem Mass SpectrometryChromatography, LiquidProteomeSecretory VesiclesPlant ProteinsProteomeLC-MS/MSPlant cellsProteomic analysisVesicle proteins

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.