Evidence map›Paper›PMID 39115670›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Thermodynamic Characterization of LC3/GABARAP:Ligand Interactions by Isothermal Titration Calorimetry.

Johannes Dopfer, Martin P Schwalm, Stefan Knapp, Vladimir V Rogov

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Johannes DopferInstitute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt, Germany.
Martin P SchwalmInstitute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt, Germany.
Stefan KnappInstitute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt, Germany.
Vladimir V RogovInstitute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt, Germany. rogov@pharmchem.uni-frankfurt.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Isothermal titration calorimetry (ITC) is a widely used technique for the characterization of protein-protein and protein-ligand interactions. It provides information on the stoichiometry, affinity, and thermodynamic driving forces of interactions. This chapter exemplifies the use of ITC to investigate interactions between human autophagy modifiers (LC3/GABARAP proteins) and their interaction partners, the LIR motif-containing sequences. The purpose of this report is to present a detailed protocol for the production of LC3/GABARAP-interacting LIR peptides using E. coli expression systems. In addition, we outline the design of ITC experiments using the LC3/GABARAP:peptide interactions as an example. Comprehensive troubleshooting notes are provided to facilitate the adaptation of these protocols to different ligand-receptor systems. The methodology outlined for studying protein-ligand interactions will help to avoid common errors and misinterpretations of experimental results.

Indexed as

Adaptor Proteins, Signal TransducingApoptosis Regulatory ProteinsCalorimetryMicrotubule-Associated ProteinsProtein BindingThermodynamicsEscherichia coliHumansLigandsPeptidesAdaptor Proteins, Signal TransducingApoptosis Regulatory ProteinsGABARAP protein, humanLigandsMAP1LC3A protein, humanMicrotubule-Associated ProteinsPeptidesAutophagyGABARAPIsothermal titration calorimetryKd determinationLC3LIR motifs

Identifiers

PMID39115670

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.