ArticleMethods in molecular biology (Clifton, N.J.)2024
Methods for Monitoring Mitophagy Using mt-Keima.
Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Mitochondria at the heart of aging: structure, function, and failure.Journal of translational medicine · 2026Review
- ALKBH1 Drives Tumorigenesis and Drug Resistance via tRNA-decoding Reprogramming and Codon-biased Translation.Cancer discovery · 2025Article
- pH-Induced Conformational Change of the Chromophore of the Large Stokes Shift Fluorescent Protein tKeima.Molecules (Basel, Switzerland) · 2025Article
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Authors and funding
2 authors.
Funding
Abstract
Mitochondria-targeted Keima (mt-Keima) is a pH-sensitive, acid-stable fluorescent protein used for the quantification of mitophagy. Mt-Keima contains a mitochondrial matrix targeting sequence and has bimodal excitation with peaks at 440 nM in neutral environments and 586 nM in acidic environments. From this bimodal excitation, a ratiometric signal may be calculated to quantify mitophagy in live cells. This chapter describes procedures for measuring mitophagy by flow cytometry and live cell confocal microscopy with mt-Keima.
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Registered trials
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