ArticleBiology of reproduction2024
Transcriptional profiling of mucus production in rhesus macaque endocervical cells under hormonal regulation†.
Article in Biology of reproduction, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Optimal Timing for Intrauterine Device Insertion During the Menstrual Cycle: A Comparative Study of Pain, Bleeding, and Postprocedural Outcomes.Clinics and practice · 2026Article
- Therapeutic potential of natural products in polycystic ovary syndrome.Molecular biology reports · 2026Review
- An Observation of the Microstructure of Cervical Mucus in Cows during the Proestrus, Estrus, and Metestrus Stages and the Impact on Sperm Penetration Ability.Veterinary sciences · 2024Article
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Authors and funding
10 authors.
Funding
Abstract
objectiveEndocervical mucus production is a key regulator of fertility throughout the menstrual cycle. With cycle-dependent variability in mucus quality and quantity, cervical mucus can either facilitate or block sperm ascension into the upper female reproductive tract. This study seeks to identify genes involved in the hormonal regulation of mucus production, modification, and regulation through profiling the transcriptome of endocervical cells from the non-human primate, the rhesus macaque (Macaca mulatta).
interventionWe treated differentiated primary endocervical cultures with estradiol (E2) and progesterone (P4) to mimic peri-ovulatory and luteal-phase hormonal changes. Using RNA-sequencing, we identified differential expression of gene pathways and mucus-producing and mucus-modifying genes in cells treated with E2 compared to hormone-free conditions and E2 compared to E2-primed cells treated with P4.
main outcome measuresWe pursued differential gene expression analysis on RNA-sequenced cells. Sequence validation was done using quantitative PCR (qPCR).
resultsOur study identified 158 genes that show significant differential expression in E2-only conditions compared to hormone-free control and 250 genes that show significant differential expression in P4-treated conditions compared to E2-only conditions. From this list, we found hormone-induced changes in transcriptional profiles for genes across several classes of mucus production, including ion channels and enzymes involved in post-translational mucin modification that have not previously been described as hormonally regulated.
conclusionOur study is the first to use an in vitro culture system to create an epithelial cell-specific transcriptome of the endocervix. As a result, our study identifies new genes and pathways altered by sex steroids in cervical mucus production. SUMMARY SENTENCE: In vitro hormonal regulation of mucus production, modification, and secretion was profiled using primary epithelial endocervical cells.
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