Evidence map›Paper›PMID 39110213›Full record

ArticleArchives of microbiology2024

Effective RNA isolation method for gram-positive and acid-fast bacteria: metamorphosed from conventional RNA isolation techniques.

Jignasa H Bera, Leyon Selvin Raj A, Hemant Kumar, Nilesh Pandey, Dhara N Patel

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In one paragraph

Article in Archives of microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Cell Disruption and RNA Extraction fromInternational journal of molecular sciences · 2026
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Jignasa H BeraDepartment of Medical Laboratory Technology, Bapubhai Desaibhai Patel Institue of Paramedical Sciences (BDIPS), Charotar University of Science and Technology, CHARUSAT campus, Changa, Anand, Gujarat, 388421, India.
Leyon Selvin Raj ADepartment of Medical Laboratory Technology, Bapubhai Desaibhai Patel Institue of Paramedical Sciences (BDIPS), Charotar University of Science and Technology, CHARUSAT campus, Changa, Anand, Gujarat, 388421, India.
Hemant KumarDepartment of Medical Laboratory Technology, Bapubhai Desaibhai Patel Institue of Paramedical Sciences (BDIPS), Charotar University of Science and Technology, CHARUSAT campus, Changa, Anand, Gujarat, 388421, India. hemantkumar.cips@charusat.ac.in.
Nilesh PandeyDepartment of Medical Laboratory Technology, Bapubhai Desaibhai Patel Institue of Paramedical Sciences (BDIPS), Charotar University of Science and Technology, CHARUSAT campus, Changa, Anand, Gujarat, 388421, India. nilesh07pandey@gmail.com.
Dhara N PatelDepartment of Medical Laboratory Technology, Bapubhai Desaibhai Patel Institue of Paramedical Sciences (BDIPS), Charotar University of Science and Technology, CHARUSAT campus, Changa, Anand, Gujarat, 388421, India. dharapatel.cips@charusat.ac.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The RNA-based study provides an excellent indication of an organism's gene expression profile. Obtaining high-yield and high-purity RNA from Gram-positive and acid-fast bacteria is difficult without high-end kits and facilities. We optimised effective and simple protocol for RNA isolation that is a combination of enzymatic, physical and chemical treatment to disrupt cells. We successfully isolated high quality intact total RNA with yields ranging from 23.13 ± 0.40 to 61.51 ± 0.27 µg and the 260/280 purity ratio of 1.95 ± 0.01 to 2.05 ± 0.01 from Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, and Mycobacterium smegmatis. These results represents a significantly enhanced yield and purity compared to other combination of techniques which we performed. Compared to previous studies the yield obtained by this method is high for the studied organisms. Furthermore the yielded RNA was successfully used for downstream applications such as quantitative real time PCR. The described method can be easily optimised and used for various bacteria.

Indexed as

RNA, BacterialEnterococcus faecalisGram-Positive BacteriaMycobacterium smegmatisStaphylococcus aureusStaphylococcus epidermidisRNA, BacterialAcid-fast bacteriaGram-positive bacteriaHard-to-lyseQuantitative real-time PCRRNA isolation

Identifiers

PMID39110213

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.