Evidence map›Paper›PMID 39109912›Full record

ArticlePediatric pulmonology2024

Detection of the epidemic Pseudomonas aeruginosa AUST-03 (ST242) strain in people with cystic fibrosis in South Africa.

Thabo Hamiwe, Debbie A White, Stanford Kwenda, Arshad Ismail, Susan Klugman, Lore Van Bruwaene, Ameena Goga, Marleen M Kock, Anthony M Smith, Marthie M Ehlers

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Article in Pediatric pulmonology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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0cells of the map it votes in
3citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Thabo HamiweDepartment of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.ORCID 0000-0002-1792-7975
Debbie A WhiteDepartment of Paediatric Pulmonology, Charlotte Maxeke Johannesburg Academic Hospital, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.ORCID 0000-0002-0943-5970
Stanford KwendaDivision of the National Health Laboratory Service, Sequencing Core Facility, National Institute for Communicable Diseases, Johannesburg, South Africa.ORCID 0000-0002-9008-4898
Arshad IsmailDivision of the National Health Laboratory Service, Sequencing Core Facility, National Institute for Communicable Diseases, Johannesburg, South Africa.ORCID 0000-0003-4672-5915
Susan KlugmanDepartment of Paediatric Pulmonology, Charlotte Maxeke Johannesburg Academic Hospital, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
Lore Van BruwaeneDepartment of Paediatric Pulmonology, Steve Biko Academic Hospital/University of Pretoria, Pretoria, South Africa.
Ameena GogaDepartment of Paediatric Pulmonology, Steve Biko Academic Hospital/University of Pretoria, Pretoria, South Africa.
Marleen M KockDepartment of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.ORCID 0000-0002-1768-9268
Anthony M SmithDepartment of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.ORCID 0000-0002-3637-7155
Marthie M EhlersDepartment of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.ORCID 0000-0003-2199-6970

Funding

Fleming FundNational Health Laboratory Service Trust 004_94726
6 · The paper itself

Abstract

introductionPseudomonas aeruginosa AUST-03 (ST242) has been reported to cause epidemics in people with CF (pwCF) from Australia and has been associated with multidrug resistance and increased morbidity and mortality. Here, we report an epidemic P. aeruginosa (AUST-03) strain in South African pwCF detected at a public hospital and characterize the genomic antibiotic resistance determinants.

methodsThe P. aeruginosa AUST-03 (ST242) study isolates were analysed with whole genome sequencing using the Illumina NextSeq2000 platform. Raw sequencing reads were processed using the Jekesa pipeline and multilocus sequence typing and genomic antibiotic resistance characterization was performed using public databases. Genetic relatedness between the study isolates and global P. aeruginosa ST242 from public databases was determined using a maximum-likelihood phylogenetic tree. Antibiotic susceptibility testing was performed using the disk diffusion and broth microdilution techniques.

resultsA total of 11 P. aeruginosa AUST-03 isolates were isolated from two children with CF. The majority (8/11) of these isolates were multidrug-resistant (MDR) or extensively drug resistant; and the multidrug efflux pumps MexAB-OprM, MexCD-OprJ, MexEF-OprN, and MexXY-OprM were the most clinically relevant antibiotic resistance determinants and were detected in all of the isolates. The study isolates were the most closely related to a 2020 P. aeruginosa AUST-03 (ST242) CF isolate from Russia.

conclusionEpidemic MDR P. aeruginosa strains are present at South African public CF clinics and need to be considered when implementing segregation and infection control strategies to prevent possible spread and outbreaks.

Indexed as

Cystic FibrosisDrug Resistance, Multiple, BacterialMicrobial Sensitivity TestsPseudomonas aeruginosaPseudomonas InfectionsAnti-Bacterial AgentsChildChild, PreschoolEpidemicsFemaleHumansMaleMultilocus Sequence TypingPhylogenySouth AfricaWhole Genome SequencingAnti-Bacterial Agentsantibiotic resistancecystic fibrosisPseudomonas aeruginosa AUST‐03South Africa

Identifiers

PMID39109912
PMCPMC11600992

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.