ArticleApplied biochemistry and biotechnology2025
Eco-Friendly Synthesis, Characterization, and Biomedical Applications of Biosynthesized Bimetallic Silver-Gold Nanoparticles by Culture Supernatant of Aspergillus niger.
Article in Applied biochemistry and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Current challenges and future directions in the green synthesis of nanoparticles using fungi.Bioprocess and biosystems engineering · 2026Article
- The effectiveness of AgNPs synthesized using Aspergillus floccosus for controlling Phoma herbarum and Aphis Pomi de Geer.World journal of microbiology & biotechnology · 2026Article
- Fungicidal efficacy of green-synthesized organosolv lignin-capped silver-based nanoparticles against amphotericin B-resistant Candida tropicalis.Folia microbiologica · 2026Article
- Biogenic Synthesis and Nematicidal Potential of Silver Nanoparticles Derived from Indigenous Microbes of Vietnam's Central Highlands.Applied biochemistry and biotechnology · 2025Article
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5 authors.
Funding
Abstract
Green synthesis of bimetallic nanoparticles of noble metals is highly desirable in nanomedicine because of their potential use as anticoagulant, thrombolytic and anticancer agents. In this study, it was discovered that the filamentous fungus Aspergillus niger proved effective in producing bimetallic Ag-Au nanoparticles. A. niger culture supernatant was able to produce Ag-AuNPs by reducing the solution of chloroauric acid/silver nitrate (1.0:1.0 mM) within 2 min at 100 °C and pH 8. Experimental Ag-AuNP detection was performed by visually observing the color change to reddish brown. The produced nanoparticles displayed maximal absorbance at 530 nm in UV-vis spectroscopy. According to transmission electron microscopy, most of the nanoparticles were spherical, with a mean diameter of 8-10 nm. The biosynthesis of Ag-AuNPs by A. niger was confirmed by Fourier transform infrared spectroscopy, X-ray diffraction and energy dispersive X-ray analytical techniques. Its zeta potential was discovered to be -34.01 mV. The biosynthesized Ag-AuNPs exhibited effective thrombolytic and antiplatelet aggregation actions by totally preventing and dissolving the blood clot which was verified by microscopic examination, amelioration of blood coagulation assays, and carrageenan-induced tail thrombosis model. The findings verified the effectiveness of biosynthesized Ag-AuNPs as a powerful antitumor agent against HepG2 and A549 cell lines with IC
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