Evidence map›Paper›PMID 39105940›Full record

ArticleJournal of molecular histology2024

Analysis of histomorphology and SERNINA5 gene expression in different regions of epididymis of cattleyak.

Haiyan Li, Cheng Pan, Yifei Wang, Jingjing Li, Zhenzhen Zhang, Khuram Shahzad, Shehr Bano Mustafa, Ye Wang, Wangsheng Zhao

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of molecular histology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Haiyan Li *College of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621000, China.
Cheng Pan *College of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621000, China.
Yifei WangDepartment of Clinical Laboratory, Sichuan Mianyang 404 Hospital, Mianyang, Sichuan, 621000, China.
Jingjing LiCollege of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621000, China.
Zhenzhen ZhangCollege of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621000, China.
Khuram ShahzadDepartment of Biosciences, COMSATS University Islamabad, Park Road, Islamabad, 45550, Pakistan.
Shehr Bano MustafaCollege of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621000, China.
Ye WangChengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, 610057, China. yewang_faith@hotmail.com.
Wangsheng ZhaoCollege of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621000, China. wangshengzhao01@163.com.

Funding

National Natural Science Foundation of China 31601946Qinghai Provincial Central Government Guidance Local Science and Technology Development Fund Project 2023ZY026Sichuan Science and Technology Program 2021YFH0101
6 · The paper itself

Abstract

The molecular mechanism of sterility in cattleyak is still unresolved. The related factors of infertility in cattleyak were studied by tissue section, SERPINA5 gene cloning and bioinformatics technology. Tissue sections of the epididymis showed poorly structured and disorganized epithelial cells in the corpus of the epididymis compared to the caput of the epididymis, while in the cauda part of the epididymis, the extra basal smooth muscle was thinner, the surface of the epithelial lumen was discontinuous and the epithelium was markedly degenerated. The results of gene cloning showed that the coding sequence (CDS) region of the SERPINA5 gene in cattleyak was 1215 bp in length, encoding a total of 404 amino acids, of which the isoleucine content was the highest, accounting for a total of 49 amino acids (12.1%). The results of real-time fluorescence quantitative PCR (qPCR) showed that the expression of the SERPINA5 gene in the epididymis caput in cattleyak was significantly higher than that in the corpus and cauda (P < 0.05), but there were no significant differences between the corpus and cauda. In the current study, histological and bioinformatics analysis, physicochemical properties, and the expression analysis of the SERPINA5 gene in different regions of the epididymis in cattleyak were carried out to explore the biological complications of cattleyak infertility.

Indexed as

EpididymisAnimalsCattleCloning, MolecularComputational BiologyGene ExpressionGene Expression RegulationMaleSerpinsSerpinsBioinformatics analysisCattleyakEpididymisSERPINA5 geneTissue section

Identifiers

PMID39105940

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.