Evidence map›Paper›PMID 39105925›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Caco-2 Monolayer as a Model of the Intestinal Barrier: Permeability of Magnesium Salts.

Nikola Chomanicova, Adriana Adamickova, Simona Valaskova, Andrea Gazova, Jan Kyselovic

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Nikola ChomanicovaInternational Laser Centre, Slovak Centre of Scientifc and Technical Information, Bratislava, Slovakia.
Adriana AdamickovaFaculty of Medicine, 5th Department of Internal Medicine, Comenius University Bratislava, Bratislava, Slovakia.
Simona ValaskovaInternational Laser Centre, Slovak Centre of Scientifc and Technical Information, Bratislava, Slovakia.
Andrea GazovaFaculty of Medicine, Institute of Pharmacology and Clinical Pharmacology, Comenius University Bratislava, Bratislava, Slovakia.
Jan KyselovicFaculty of Medicine, 5th Department of Internal Medicine, Comenius University Bratislava, Bratislava, Slovakia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Magnesium, an essential mineral for various physiological functions, is subject to tight regulation within the body. Understanding its absorption across epithelial cell monolayers is crucial for optimizing dietary magnesium intake and therapeutic strategies. The Caco-2 monolayer model, widely recognized for its relevance to the human intestinal epithelium, provides a suitable platform for this investigation. This protocol covers the step-by-step procedures for the cultivation of Caco-2 monolayer preparation of transwell systems. It provides guidance on the setup of magnesium transport experiments, which involve the application of magnesium salts to the apical side of the Caco-2 monolayer and monitoring their transport to the basolateral side.

Indexed as

Intestinal MucosaMagnesiumBiological TransportCaco-2 CellsCell Culture TechniquesHumansIntestinal AbsorptionPermeabilitySaltsMagnesiumSaltsCaco-2 cell lineCaco-2 monolayersIntestinal barrierMagnesium absorptionMagnesium saltsMagnesium transport

Identifiers

PMID39105925

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.